Assessment of morphological and molecular diversity and genetics of resistance to bacterial leaf blight and mosaic virus in cowpea (vigna unguiculata L. walp.)
KrishiKosh
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Title |
Assessment of morphological and molecular diversity and genetics of resistance to bacterial leaf blight and mosaic virus in cowpea (vigna unguiculata L. walp.)
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Creator |
Khan, Hasan
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Contributor |
K. P, Viswanatha
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Subject |
cowpeas, planting, genotypes, diseases, genetics, sowing, developmental stages, yields, biological phenomena, crossing over
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Description |
An investigation was undertaken with 196 cowpea genotypes to study morphological and molecular diversity using SSR markers and field screening of mosaic virus and Bacterial leaf blight disease for the identification of DNA markers linked to BLB. Analysis of variance revealed significant difference for all the nine quantitative traits. The estimates of PCV and GCV were high for plant height, number of branches plantˉ1, pods plantˉ1, pod length, test weight and seed yield plantˉ1. High heritability and genetic advance was observed for plant height, number of branches plantˉ1, pods plantˉ1 pod length, seeds pod-1, test weight and seed yield. Genetic divergence study using Mahalanobis D2 statistic grouped 196 genotypes into 22 clusters. Cluster XXII was the largest comprising of 133 genotypes followed by cluster I with 23 genotypes, and all other clusters had only two genotypes each. Intra cluster distance was highest in the cluster XXII followed by the cluster I and cluster XXI. The genotypes included are found to be very diverse in nature as they showed maximum inter cluster distance (D2) between the clusters I and XIX, the minimum D2 value was between the clusters II and VI. Molecular characterization with 60 genotypes revealed at 65 per cent similarity, seven clusters were formed and II cluster had only one genotype i.e. EC 472252 and cluster V largest with 28 genotypes. The genotypes found resistant in field screening of 196 entries were subjected to artificial inoculation for both diseases and resistant C-152 susceptible for both diseases and V-16, HC-03-02 were found resistance to BLB and CpMV diseases, respectively. Inheritance studies for CpMV and BLB diseases involving two crosses of susceptible with resistant parents revealed the role of single dominant gene for both the diseases. Only three SSR markers were polymorphic out of 12 markers studied for BLB resistance in the BSA of F2 cross (C-152 x V-16). Markers CP-641/642 showed significant linkage on the basis of single factor analysis of these three putative markers, which leads to conclude for the possible linkage between the markers and BLB resistance gene. |
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Date |
2016-05-17T13:23:04Z
2016-05-17T13:23:04Z 2012-03-17 |
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Type |
Thesis
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Identifier |
Th-10199
http://krishikosh.egranth.ac.in/handle/1/66022 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences, Bengaluru
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