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Assessment of morphological and molecular diversity and genetics of resistance to bacterial leaf blight and mosaic virus in cowpea (vigna unguiculata L. walp.)

KrishiKosh

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Title Assessment of morphological and molecular diversity and genetics of resistance to bacterial leaf blight and mosaic virus in cowpea (vigna unguiculata L. walp.)
 
Creator Khan, Hasan
 
Contributor K. P, Viswanatha
 
Subject cowpeas, planting, genotypes, diseases, genetics, sowing, developmental stages, yields, biological phenomena, crossing over
 
Description An investigation was undertaken with 196 cowpea genotypes to study
morphological and molecular diversity using SSR markers and field screening of mosaic
virus and Bacterial leaf blight disease for the identification of DNA markers linked to
BLB. Analysis of variance revealed significant difference for all the nine quantitative
traits. The estimates of PCV and GCV were high for plant height, number of branches
plantˉ1, pods plantˉ1, pod length, test weight and seed yield plantˉ1. High heritability and
genetic advance was observed for plant height, number of branches plantˉ1, pods plantˉ1
pod length, seeds pod-1, test weight and seed yield. Genetic divergence study using
Mahalanobis D2 statistic grouped 196 genotypes into 22 clusters. Cluster XXII was the
largest comprising of 133 genotypes followed by cluster I with 23 genotypes, and all
other clusters had only two genotypes each. Intra cluster distance was highest in the
cluster XXII followed by the cluster I and cluster XXI. The genotypes included are
found to be very diverse in nature as they showed maximum inter cluster distance (D2)
between the clusters I and XIX, the minimum D2 value was between the clusters II and
VI. Molecular characterization with 60 genotypes revealed at 65 per cent similarity,
seven clusters were formed and II cluster had only one genotype i.e. EC 472252 and
cluster V largest with 28 genotypes. The genotypes found resistant in field screening of
196 entries were subjected to artificial inoculation for both diseases and resistant C-152
susceptible for both diseases and V-16, HC-03-02 were found resistance to BLB and
CpMV diseases, respectively. Inheritance studies for CpMV and BLB diseases involving
two crosses of susceptible with resistant parents revealed the role of single dominant gene
for both the diseases. Only three SSR markers were polymorphic out of 12 markers
studied for BLB resistance in the BSA of F2 cross (C-152 x V-16). Markers CP-641/642
showed significant linkage on the basis of single factor analysis of these three putative
markers, which leads to conclude for the possible linkage between the markers and BLB
resistance gene.
 
Date 2016-05-17T13:23:04Z
2016-05-17T13:23:04Z
2012-03-17
 
Type Thesis
 
Identifier Th-10199
http://krishikosh.egranth.ac.in/handle/1/66022
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru