Isolation and screening of efficient algae for biofuel production
KrishiKosh
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Title |
Isolation and screening of efficient algae for biofuel production
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Creator |
Jyoti Rai
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Contributor |
Gosal, S. K.
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Subject |
biological phenomena
biofuel . |
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Description |
A total of 20 different algal isolates were isolated from stagnant water samples collected from different districts of Punjab, Rajasthan and Himachal Pradesh. On the basis of morphological characterization, these algal isolates were tentatively identified as Chlorella, Oedogonium, Aphanocapsa, Phormidium, Spirogyra, Anabeana, Scenedesmus, Tolypothrix, Anacystis, Microcystis, Volvox and Spirulina. Data analysis revealed that the isolate JA8 had highest growth rate followed by JA12 after 16 days of incubation. Increase in pH of the medium was recorded with the growth of algal isolates and increase in pH in different algal isolates ranged from 7.0 (initial) to 10.6 (final) during algal growth after 16 days of incubation. The highest increase in pH was recorded in the JA18 (10.6) which were significantly higher than control (7.0). The supplementation of urea to the growth medium did not result in much increase in pH as compared to the medium which was not supplemented with urea. The increase in pH with urea supplementation ranged from 7.0 to 8.2 whereas without urea supplementation the pH increased upto 10.6. The isolate JA11 showed highest fresh weight (16.284 g/l) and dry weight (7.211 g/l) of biomass after one month of incubation. All the 20 isolates, were screened for their lipid content and the highest lipid content was recorded in the isolate JA8 (5.60 %) followed by JA9 (5.25 %). The addition of glucose (10 g/l) to the growth medium resulted in increase in lipid content in the isolate JA8 (5.60 % to 7.52 %) and JA9 (5.25% to 7.15%). Saponification and acid value of isolates was analyzed using five best screened isolates based on their lipid content. The isolate JA8 showed highest saponification (169.69 mg/g of oil) and acid value (4.75 mg/g of oil) followed by the isolate JA9. The fatty acid composition of three algal isolates JA8, JA9 and JA15 was determined with Gas liquid Chromatography. The isolate JA8 contained highest proportion of stearic acid (41.60), followed by pentadecanoic acid (38.32) and oleic acid (19.01). Isolate JA9 and JA15 contained saturated fatty acids only. Hence, the results revealed that the isolate JA8 may be considered best among the isolates isolated making it suitable for biofuel production. However, the lipid content of the isolates is not much promising. So, there is need to explore the possibility to find algal isolate which is having high lipid content for c.
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Date |
2017-01-04T13:48:19Z
2017-01-04T13:48:19Z 2013 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/94428
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Language |
en
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Format |
application/pdf
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Publisher |
PUNJAB AGRICULTURAL UNIVERSITY
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