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IDENTIFICATION OF DNA MARKERS ASSOCIATED TO GRAIN PROTEIN CONTENT IN RICE (Oryza sativa L.)

KrishiKosh

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Title IDENTIFICATION OF DNA MARKERS ASSOCIATED TO GRAIN PROTEIN CONTENT IN RICE (Oryza sativa L.)
M.Sc. Biotechnology
 
Creator Dadsena, Balwant
 
Contributor Chandel, Girish
 
Subject DNA MARKERS, GRAIN PROTEIN CONTENT, RICE (Oryza sativa L.), Biotechnology
 
Description Plant source of protein plays a vital role in human health and shown benefits in improving the every malnutrition condition. Rice (Oryza sativa L.) a major staple food of one third world’s population serves basis of food for peoples. The improvement in grain protein content (GPC) and its composition in protein has been a major concern of plant breeders. It has been difficult to achieve for effective selection criteria and because selection is expensive and time-consuming. Identification of molecular markers associated with grain protein content of rice will easy and effective approach for selection and identification of high Grain protein content (GPC) rice genotypes. Hence the present study was undertaken with the 76 rice diverse rice genotypes in order to find out the molecular markers associated with GPC of rice.
Initially 76 rice were characterized for 17 morphological traits, which includes 8 quantitative and 9 qualitative traits. Wide variation for all the 17 traits observed as expected. Wide variability was observed in 8 quantitative traits and 9 morphological traits along with the GPC of rice which was evidenced by the high range and mean performance in 76 genotypes. On the basis of the mean performance of the quantitative trait the genotype R-RHZ-IB-13 (high zinc line) has been identified as a line of interest based on desirable plant type viz., short plant height (99cm), early days to fifty percent flowering(85.5 days), and more tiller number (8.2), long panicle length (24.33 cm), long grain length (9.4 mm). Similarly based on the GPC value high GPC containing germplasm lines namely -154 136 (10.28%), Tulsi gurmatia (10.06%) , Bada gada khuta (9.94%), RKVY-52 (9.94%), GP-145-65 (9.82%), GP-145-59 (9.78%), GP, were identified which will be further used as donor lines for this trait in breeding programmes. Correlation study showed that there was no significant association was between GPC and other quantitative traits hence based on these traits value identification of high GPC line can’t be identified.
Total 37 microsatellites markers were used for this study out of only 14 markers found to be polymorphic. These polymorphic set were used for the genetic analysis of 76 genotypes. Overall 28 alleles generated by the polymorphic markers set among them RM21 (0.499) showed maximum PIC value. Association analysis revealed that out of 14 SSR makers only 5 markers RM1, RM452, RM234, RM160 and RM21found to be associated with GPC of rice which is present on chromosome number 1, 2, 7, 9 and 11. Further these associated marker regions were mined for the protein specific candidates genes present in the 20 kb upstream and downstream region and analysis revealed that total of 22 genes present in the associated region. These associated marker set will be further used for the marker assisted selection for high GPC lines and candidate genes identified should be validated for their presence and expression variability in high and low GPC lines.
 
Date 2016-08-17T11:16:46Z
2016-08-17T11:16:46Z
2016
 
Type Thesis
 
Identifier 110p.
http://krishikosh.egranth.ac.in/handle/1/72702
 
Language en
 
Format application/pdf
 
Publisher Indirra Gandhi Krishi Vishwavidyalaya Raipur