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STUDY OF SSR POLYMORPHISM FOR BIOTIC STRESS RESISTANCE IN RICE

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Title STUDY OF SSR POLYMORPHISM FOR BIOTIC STRESS RESISTANCE IN RICE
 
Creator SHIVA KUMAR GANIWADA
 
Contributor DURGA RANI, Ch.V
 
Subject cotton, genetics, genotypes, yields, sowing, gossypium hirsutum, biological phenomena, planting, selection, heritability
 
Description Rice (Oryza sativa L.) is the most important food crop in the world and feeds more
than half of the global population. The productivity of rice is being affected by a number of
insect pests, bacterial, fungal and viral diseases. The development of host-plant resistance is
considered as the most economical, effective means to control the important pests and
diseases. In order to achieve durable resistance, gene pyramiding or gene stacking or both
together is advisable to develop new rice varieties with multiple pest and disease resistance
or to improve the existing high yielding varieties (HYVs) and hybrids by introgressing the
genes for pests and diseases. With the advent of molecular markers, marker assisted
selection or marker assisted back cross breeding allows the identification of plants carrying
multiple resistance genes through foreground and background selection.
A research project has been initiated in the department of Agricultural
Biotechnology to introgress four resistance genes (xa13, Xa21, Pi-kh and Gm4) into the
genetic background of a popular rice variety Jagtial Sannalu (JGL 1798). The present
investigation was aimed to survey the SSR polymorphism between parents linked to the
four biotic stress resistance genes viz., xa13, Xa21, Pi-kh and Gm4 and also to screen with
hyper variable rice SSR markers to study the polymorphism between recipient parent,
Jagtial Sannalu (JGL 1798) and donor parents (GPP1 and NLR 145) in order to identify
markers for use in foreground as well as background selection. Three gene specific primer
pairs viz., xa13 promoter, pTA248 and RM547 that are closely linked to the resistance genes
viz., xa13 (BLB), Xa21(BLB) and Gm4 (Gall midge), respectively were validated by
comparing with the check materials, B 95-1 for BLB and Abhaya for gall midge resistance
xiii
genes. RM 206 is another primer pair that is closely linked to blast resistance Pi-kh was
validated by comparing with that of Tetep, the source material for Pi-kh gene. The target
genes xa13 and Gm4 are located on chromosome 8, while Xa21 and Pi-kh genes are located
on chromosome11.
Once the validation was done for the target genes, the SSR polymorphism for the
parental lines (recurrent and donor parents) with respect to the target resistance genes was
done. The results revealed that clear polymorphism was observed between the donor and
recipient parents for susceptible and resistant alleles for all the target genes.
One hundred and twenty eight hyper variable SSR markers were analyzed in three
parental lines (JGL 1798, GPP1 and NLR 145) to assess the polymorphism between
parental lines in order to use them in background selection. Among the 128 primer pairs,
92% primers (118) showed clear amplification pattern in all the three genotypes. Thirty six
primer pairs demonstrated polymorphism among the parental lines. Out of 36 polymorphic
primer pairs, a maximum of seven primer pairs were observed on chromosome 3, followed
by four primer pairs on chromosome 4, three each on chromosomes 1, 2, 6, 8, 9 and 12, two
each on chromosomes 5, 7 and 11 and one on chromosome number 10. Among 36 primer
pairs, AT repeats were maximum in number (18) followed by 14 AAT, 2 AGAT and 2 AC
repeats. Twenty two primer pairs viz., HRM 10936, HRM 10167, HRM 11111, HRM
13659, HRM 14250, HRM 15855, HRM 16153, HRM 15679, HRM 17405, HRM 16652,
HRM 18939, HRM 20583, HRM 20710, HRM 21881, HRM 22622, HRM 23146, HRM
23578, HRM 25310, HRM 27323, HRM 28110, HRM 28202, and HRM 28800 were
detected as polymorphic in nature between JGL 1798 and GPP1 (B 95-1 × Abhaya).
Thirty one primer pairs viz., HRM 10167, HRM 11111, HRM 13154, HRM 12469,
HRM 13659, HRM14250, HRM 15626, HRM 15831, HRM 15855, HRM 16153, HRM
15337, HRM 17405, HRM 16913, HRM 16652, HRM 16649, HRM 18939, HRM 18770,
HRM 19697, HRM 21539, HRM 21881, HRM 22622, HRM 23146, HRM 23578, HRM
24481, HRM 24542, HRM 24199, HRM 27323, HRM 25970, HRM 28110, HRM 28202
and HRM 28800 were observed as polymorphic markers between JGL 1798 (Jagtial
Sannalu) and NLR 145 (Swarnamukhi).
 
Date 2016-06-06T11:40:48Z
2016-06-06T11:40:48Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66821
 
Language en
 
Relation D8812;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY