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Molecular characterisation of root specific mapping population of mulberry by SSR markers and identification of QTLs Governing drought tolerance traits

KrishiKosh

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Title Molecular characterisation of root specific mapping population of mulberry by SSR markers and identification of QTLs Governing drought tolerance traits
 
Creator Jyoti, Biradar
 
Contributor T. K, Narayanaswamy
 
Subject genomes, genetic structures, genetics, dna, planting, polymorphism, genotypes, locus, breeds (animals), chromosome mapping
 
Description Mulberry leaf is the only food source for the silkworm Bombyx mori L, hence drives the
silk industry in India. There is an intimate connection between Mulberry and the
silkworm. India is the second largest silk producer by contributing to 18% of the world
silk requirement. But, the industry faces major constraints between demand and supply
due to the lack of improved varieties. The advancement of sericulture industry depends
upon the quantitative and qualitative improvement of mulberry varieties. It is strongly
opined that application of genomic tools would be mandatory to achieve this. DNA based
markers have proved to be the choice of the system. Among them SSRs found to be more
promising because of their co-dominance, multiallelic capabilities. Though SSR markers
are potential, their availability in mulberry is very much limited. The present study
focused to develop more number of SSR markers in mulberry and construct a linkage
map for molecular breeding applications. Pre cloning enrichment strategy was adopted
for the marker development. A total of 222 genomic and 136 genic markers were
developed out of 126 Mb of the mulberry genome. Out of 358 markers, 188 were found
locus specific and were characterized in diverse mulberry species and genotypes. The
transferability of these markers was also assessed in three other related species belonging
to the family Moraceae viz., Ficus, Fig and Jackfruit. All the markers showed single
locus amplification in morus species and genotypes and 79.25% were transferable to
cross species. The phylogenetic relationship of the 14 mulberry species and genotypes
belonging to the family Moraceae were revealed according to their evolutionary
pedigree.
The locus specific markers were further used for mapping using 150 F1
population generated by the cross between high rooting genotype Dudia white and low
rooting genotype UP105. The linkage map was constructed based on the segregation
pattern of the markers using Mapmaker V 3.0 software. A total of 82 out of 150 markers
segregating in 1:1 ratio could aligned in 14 linkage groups covering 3330.9cM of the
mulberry genome. All the linked markers were randomly aligned over the linkage groups
accounting an average of 40.6cM between two markers. Of the 14 linkage gropus LG1
and LG4 were dense with maximum number of markers. The linkage map was further
used to identify markers associated with quantitative trait loci (QTL) for rooting traits in
mulberry. The segregating population was screened for four quantitative traits
contributing to root and water use efficiency and their association with the linked
markers. The mapping population showed a normal distribution pattern for all the traits.
Significant association between marker and traits were established for root length,
root weight, total leaf area and total dry matter by single marker regression (SMR) on the
basis of analysis of variance (ANOVA) at p
 
Date 2016-05-21T10:18:59Z
2016-05-21T10:18:59Z
2013-09-20
 
Type Thesis
 
Identifier Th-10655
http://krishikosh.egranth.ac.in/handle/1/66191
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru