Evaluation Of Bull Semen Quality In Relation To Fertility Associated Proteins, Lipid Peroxidation And In Vitro Sperm Characters
KrishiKosh
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Title |
Evaluation Of Bull Semen Quality In Relation To Fertility Associated Proteins, Lipid Peroxidation And In Vitro Sperm Characters
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Creator |
Karunakaran, M
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Contributor |
Devanathan, T.G.
Kulasekar, K Sridevi, P Jawahar, K. Thilak Pon |
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Subject |
Bull semen
fertility associated proteins lipid peroxidation sperm characters |
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Description |
Fresh semen samples were collected from 22 breeding bulls to screen for the presence of fertility associated proteins. Seminal plasma proteins revealed 15 numbers of protein bands. The fertility related proteins such as 15/14 kDa and 28 kDa were present in all the 22 bulls (100 %), while 26 kDa protein was present in 18 bulls (81.82 %) and 55 kDa protein was present in 14 bulls (63.3 %). SDS- PAGE of sperm membrane protein revealed a total of 14 protein bands. Proteins related with bull fertility such as 15/14 kDa protein was observed in all the 22 bulls (100 %), 28 kDa protein was present in 21 bulls (95.45 %), 26 kDa protein was present in 14 bulls (63.64 %) and 55 kDa protein was present in only 11 bulls (50.00 %). Heparin binding proteins of the seminal plasma revealed 7 protein bands, the fertility related proteins such as 15/14 kDa protein was present in all the 22 bulls (100 %) and 28 kDa protein was present in 18 bulls (81.82 %). 10 protein bands were observed in the heparin binding proteins of the sperm membrane. 15/14 kDa fertility related protein was present in all the bulls, while 28 kDa protein was present in 11 bulls (50.00 %). Based on the presence of 28 kDa heparin binding protein insperm membrane, bulls in the present study were categorized into group I bulls which were positive for the protein and group II bulls negative for the protein. To study the effect of oxidative stress and role of fertility associated proteins on in vitro sperm characters, frozen thawed semen of the bulls evaluated for their in vitro sperm characters after treating with H 2 O 2 and with fertility associated proteins. In group I bulls, frozen thawed semen samples treated with 25μg of fertility associated protein had significantly (P < 0.01) lower level of MDA than the control at 60 min ((1.86 ± 0.17 vs 2.67 ± 0.19), at 120 min (2.25 ± 0.19 vs 2.79 ± 0.24) and at 180 min (2.81 ± 0.26 vs 3.77 ± 0.41).In group IIbulls, semen samples treated with fertility associated protein also had significantly lower level of MDA than the control at 60 min (2.03 ± 0.12 vs 3.04 ± 0.15), at 120 min (2.55 ± 0.13 vs 3.61 ± 0.28) and at 180 min (3.16 ± 0.14 vs 4.84 ± 0.46).In H 2 O 2 treatment, bulls in group I had significantly (P < 0.05) lower MDA level than group II bulls at 30 min of incubation (2.85± 0.16 vs 3.26 ± 0.12) and at 60 min of incubation (3.04 ± 0.16 vs 3.51 ± 0.12). Bulls in group I when treated with fertility associated proteinhad significantly more number of sperm cells with intact plasma membrane than the control at 120 min (36.02 ± 2.10 vs 28.22 ± 3.10) and at 180 min (26.69 ± 2.06 vs 15.27 ± 2.11) of incubation. Similarly, bulls in group II when treated with fertility associated protein had significantly more number of sperm cells with intact plasma membrane than the control at 120 min (32.50 ± 3.03 vs 25.96 ± 3.32) and at 180 min (23.36 ± 2.17 vs 15.06 ± 2.16) of incubation.In H 2 O 2 treated semen samples, group I bulls had significantly (P < 0.01) more number of sperm cells with intact plasma membrane than the group II bulls at 10 min (45.75 ± 3.44 vs 31.79 ± 3.34) and at 30 min(26.03 ± 2.53 vs 17.54 ± 1.59) post thaw incubation periods. Bulls in group I and II did not differ with each other in the per cent of sperm cells with HMMP at 10 min of incubation with H 2 O 2 (15.30 ± 0.92 vs 14.86 ± 0.77). When the semen samples were treated with fertility associated proteins, bulls in group I had significantly (P< 0.01) more per cent of sperm cells with HMMP than the group II during different points of incubation; 60 min (17.51 ± 0.90 vs 13.57 ±0.85), at 120 min (14.54 ± 0.58 vs 9.58 ± 0.76) and at 180 min (10.54 ± 0.55 vs 7.49 ± 0.89) incubation periods. In H 2 O 2 treated samples, group I bulls had significantly (P < 0.05) more number of DNAintact sperm cells than the group II bulls at 60 min of incubation (94.01 ± 0.51 vs 92.26 ± 0.56). In H 2 O 2 treatment, group I bulls had significantly (P < 0.01) less number of apoptotic cells (10.53 ± 1.35 vs 13.90 ± 1.52)than group II at 30 min of incubation. When the samples were treated with fertility associated protein, group I bulls had significantly (P |
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Date |
2016-05-31T11:48:19Z
2016-05-31T11:48:19Z 2011 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/66473
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Language |
en
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Format |
application/pdf
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Publisher |
Tamil Nadu Veterinary and Animal Sciences University
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