Development and Evaluation of Eimeria tenella Sporozoite Vaccine in Broiler Chickens
KrishiKosh
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Title |
Development and Evaluation of Eimeria tenella Sporozoite Vaccine in Broiler Chickens
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Creator |
Saravanan, S.
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Contributor |
Palanivel, K.M.
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Description |
In the present work on development and evaluation of E. tenella sporozoites vaccine from field isolates of caecal coccidiosis in broiler chickens, the protective immune response and efficacy of the vaccine were assessed by ELISA and IFN-? assay, and challenge studies, respectively. The purity of the sporozoite protein from the field isolates revealed the molecular weight in the range of 30, 97 and 140 KDa by SDS-PAGE which was used as a potential vaccine candidate. The experimental study was carried out with 130 day old Cobb 400 broiler chicks which were divided for two experimental trials, each comprising five treatment groups. In the experimental trial-I, the groups, T1 and T2 were administered 10 and 20 ?g of live sporozoite antigen, respectively on 2nd day of age, and T3 and T4 were administered 10 and 20 ?g of live sporozoite antigen, respectively on 6th day of age. In experimental trial-II, T1 was administered 25 ?g of live sporozoites as primary and booster doses on 2nd and 18th day of age, respectively and T2 with 25 ?g of live sporozoites with FCA, as primary vaccination on 2nd day and booster vaccination without adjuvant on 18th day of age. T3 was administered live anticoccidial vaccine (Livacox-Q?) at the recommended dosage on the 2nd day of age and the group T4 was administered the anticoccidial drug (diclazuril) via feed at the recommended dosage from 2nd day of age, till the end of experiment. Group T5 was kept as control in both the trials I and II. Humoral (IgG) and cell mediated immune response (CMI) were assessed by sporozoite specific indirect ELISA and gamma interferon assay, respectively from day 7 of life at weekly intervals. Significantly high (IgG) antibody levels (P |
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Date |
2016-07-26T15:38:17Z
2016-07-26T15:38:17Z 2013 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/69960
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Language |
en
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Format |
application/pdf
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Publisher |
Tamil Nadu Veterinary and Animal Sciences University
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