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Development and Evaluation of Eimeria tenella Sporozoite Vaccine in Broiler Chickens

KrishiKosh

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Title Development and Evaluation of Eimeria tenella Sporozoite Vaccine in Broiler Chickens
 
Creator Saravanan, S.
 
Contributor Palanivel, K.M.
 
Description In the present work on development and evaluation of E. tenella sporozoites vaccine
from field isolates of caecal coccidiosis in broiler chickens, the protective immune response
and efficacy of the vaccine were assessed by ELISA and IFN-? assay, and challenge
studies, respectively. The purity of the sporozoite protein from the field isolates revealed the
molecular weight in the range of 30, 97 and 140 KDa by SDS-PAGE which was used as a
potential vaccine candidate.
The experimental study was carried out with 130 day old Cobb 400 broiler chicks
which were divided for two experimental trials, each comprising five treatment groups. In the
experimental trial-I, the groups, T1 and T2 were administered 10 and 20 ?g of live sporozoite
antigen, respectively on 2nd day of age, and T3 and T4 were administered 10 and 20 ?g of
live sporozoite antigen, respectively on 6th day of age. In experimental trial-II, T1 was
administered 25 ?g of live sporozoites as primary and booster doses on 2nd and 18th day of
age, respectively and T2 with 25 ?g of live sporozoites with FCA, as primary vaccination on
2nd day and booster vaccination without adjuvant on 18th day of age. T3 was administered
live anticoccidial vaccine (Livacox-Q?) at the recommended dosage on the 2nd day of age
and the group T4 was administered the anticoccidial drug (diclazuril) via feed at the
recommended dosage from 2nd day of age, till the end of experiment. Group T5 was kept as
control in both the trials I and II.
Humoral (IgG) and cell mediated immune response (CMI) were assessed by
sporozoite specific indirect ELISA and gamma interferon assay, respectively from day 7 of
life at weekly intervals. Significantly high (IgG) antibody levels (P
 
Date 2016-07-26T15:38:17Z
2016-07-26T15:38:17Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69960
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University