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Local Antibody Production in the Oviduct and Gut of Hens Infected with a Variant Strain of Infectious Bronchitis Virus

KrishiKosh

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Title Local Antibody Production in the Oviduct and Gut of Hens Infected with a Variant Strain of Infectious Bronchitis Virus
 
Creator Raj, G. Dhinakar
Jones, R.C.
 
Description Following infection of 16-week old specific pathogen-free (SPF) female chickens with an enterotropic variant of infectious bronchitis virus (IBV) strain G, IBV-specific Immunoglobulin G (IgG) and IgA were detected in tears, tracheal washes, oviduch washes, duodenal and caecal contents using class-specific monoclonal antibodies in enzyme linked immunosorbent assays (ELISA). IgG antibody content was highest in tears on Day 7 post-infection (p.i.) and was still detectable on Day 23 p.i. Significant levels of IgG antibody were present in oviduct washes on Days 7 and 23 p.i. Tears showed the highest IgA antibody concentration of any sample on Day 7 p.i. but this decreased to an insignificant level by Day 17 p.i. Oviduct and tracheal washes had IgA antibodies on Day 7 p.i. Significant levels of both antibody classes were detected in duodenal contents on Day 17 p.i. only but neither was present in caecal contents. Using a methods which compares titre of antibody produced from pieces of tissue at 4°C was higher than at 37°C, suggestive ofhigh concentrations of extracellular antibody. In a separate experiment, passively administered IBV antibody transuded from serum into oviduct washes but did not do so in the trachea. In a third experiment, ELISA and haemagglutination-inhibition (HI) titres of serum and oviduct washes from ten hens previously infected when day-old were compared with egg production. Maximum positive correlation was seen between HI titres of oviduct washes and egg production.
 
Date 2016-08-10T10:12:32Z
2016-08-10T10:12:32Z
1996
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71823
 
Language en
 
Format application/pdf
 
Publisher Veterinary Immunology and Immunopathology