Evaluation Of Antibiotic Resistant Mastitis In Dairy Cows
KrishiKosh
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Title |
Evaluation Of Antibiotic Resistant Mastitis In Dairy Cows
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Creator |
Chandrasekaran, D.
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Contributor |
Nambi, A.P.
Kumanan, K. Thirunavukkarasu, P.S. Vairamuthu, S. |
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Subject |
Acute resistant mastitis
Culture E.coli S.aureus MRSA In vitro sensitivity test MIC PCR target gene Genatamicin Enrofloxacin Ceftriaxone Amoxicillin+sulbactam |
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Description |
The aim of the study was to study the prevalence of drug resistant mastitis, clinico-pathological changes and their pattern of antibiotic resistance in dairy cows. Comparative evaluation of different therapeutic protocols for clinical management of resistant mastitis and assessment of the economic impact of drug resistant mastitis were undertaken for the study. Twenty apparently healthy cows were taken as healthy control group. Based on culture and antibiotic sensitivity tests, two hundred and thirty five cases of resistant mastitis were selected and grouped as group I E.coli (n=119), group II Staphylococcus aureus (n=104) and group III methicillin resistant Staphylococcus aureus (MRSA)(n=12) and subjected to clinical examination, haematology, serum biochemistry and minimum inhibitory concentration (MIC). The incidence of clinical mastitis at Madras Veterinary College Teaching Hospital was 9.57 per cent, of which 1.90 per cent was acute mastitis and incidence in organized farm was 16.56 per cent, of which 11.08 per cent was acute mastitis. The incidence of resistant mastitis was 56.l per cent. The predominant resistant causative pathogen was E.coli (50.64 per cent) followed by S.aureus (44.25 per cent) and MRSA (5.11 per cent). Highest incidence was observed in early stage of third lactation and hind quarters. Haemato biochemical changes were reduced Hb, PCV, TEC, leukocytosis with neutrophilia, lymphopenia, hypoalbuminemia and hyperglobulinemia. A significant increase in ALP and AST were observed in early lactation which might reflect the negative energy balance and fatty liver. In vitro antibiotic sensitivity test and MIC breakpoints, E.coli, S.aureus and MRSA organisms showed more sensitivity to enrofloxacin, amoxicillin+sulbactam, gentamicin and ceftriaxone and had highest resistant to penicillin followed by amoxicillin, oxytetracycline and methicillin. Most of MRSA isolates were found to be multi-drug resistant whereas E.coli and S.aureus isolates were found to be resistant. Nitrocefin and MRSA alert kit was found to be useful in preliminary screening of β lactamase production and methicillin resistant organisms in clinical mastitis. Targeting the specific genes of E.coli, S.aureus and MRSA isolates and performing multiplex PCR were useful in the confirmation. Highly significant increase in pH and SCC and a significant decrease in electrical conductivity were noticed in all clinical mastitis. In E.coli and S.aureus mastitis treated with amoxycillin+sulbactam, ceftriaxone, enrofloxacin and gentamicin showed uniform improvement in clinical mastitis. In MRSA mastitis, enrofloxacin was found to be highly effective in comparison to amoxicillin+sulbcactam. Similar economic impact was observed in E.coli, S.aureus and MRSA mastitis. |
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Date |
2016-05-24T11:45:09Z
2016-05-24T11:45:09Z 2013 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/66263
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Language |
en
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Format |
application/pdf
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Publisher |
Tamil Nadu Veterinary and Animal Sciences University
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