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Evaluation Of Antibiotic Resistant Mastitis In Dairy Cows

KrishiKosh

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Title Evaluation Of Antibiotic Resistant Mastitis In Dairy Cows
 
Creator Chandrasekaran, D.
 
Contributor Nambi, A.P.
Kumanan, K.
Thirunavukkarasu, P.S.
Vairamuthu, S.
 
Subject Acute resistant mastitis
Culture
E.coli
S.aureus
MRSA
In vitro sensitivity test
MIC
PCR target gene
Genatamicin
Enrofloxacin
Ceftriaxone
Amoxicillin+sulbactam
 
Description The aim of the study was to study the prevalence of drug resistant mastitis,
clinico-pathological changes and their pattern of antibiotic resistance in dairy cows.
Comparative evaluation of different therapeutic protocols for clinical management of
resistant mastitis and assessment of the economic impact of drug resistant mastitis
were undertaken for the study.
Twenty apparently healthy cows were taken as healthy control group. Based
on culture and antibiotic sensitivity tests, two hundred and thirty five cases of resistant
mastitis were selected and grouped as group I E.coli (n=119), group II Staphylococcus
aureus (n=104) and group III methicillin resistant Staphylococcus aureus
(MRSA)(n=12) and subjected to clinical examination, haematology, serum
biochemistry and minimum inhibitory concentration (MIC).
The incidence of clinical mastitis at Madras Veterinary College Teaching
Hospital was 9.57 per cent, of which 1.90 per cent was acute mastitis and incidence in
organized farm was 16.56 per cent, of which 11.08 per cent was acute mastitis. The
incidence of resistant mastitis was 56.l per cent.
The predominant resistant causative pathogen was E.coli (50.64 per cent) followed
by S.aureus (44.25 per cent) and MRSA (5.11 per cent). Highest incidence was observed
in early stage of third lactation and hind quarters. Haemato biochemical changes were
reduced Hb, PCV, TEC, leukocytosis with neutrophilia, lymphopenia,
hypoalbuminemia and hyperglobulinemia. A significant increase in ALP and AST
were observed in early lactation which might reflect the negative energy balance and
fatty liver.
In vitro antibiotic sensitivity test and MIC breakpoints, E.coli, S.aureus and
MRSA organisms showed more sensitivity to enrofloxacin, amoxicillin+sulbactam,
gentamicin and ceftriaxone and had highest resistant to penicillin followed by
amoxicillin, oxytetracycline and methicillin. Most of MRSA isolates were found to be
multi-drug resistant whereas E.coli and S.aureus isolates were found to be resistant.
Nitrocefin and MRSA alert kit was found to be useful in preliminary screening of
β lactamase production and methicillin resistant organisms in clinical mastitis.
Targeting the specific genes of E.coli, S.aureus and MRSA isolates and performing
multiplex PCR were useful in the confirmation.
Highly significant increase in pH and SCC and a significant decrease in
electrical conductivity were noticed in all clinical mastitis. In E.coli and S.aureus
mastitis treated with amoxycillin+sulbactam, ceftriaxone, enrofloxacin and
gentamicin showed uniform improvement in clinical mastitis. In MRSA mastitis,
enrofloxacin was found to be highly effective in comparison to
amoxicillin+sulbcactam. Similar economic impact was observed in E.coli, S.aureus
and MRSA mastitis.
 
Date 2016-05-24T11:45:09Z
2016-05-24T11:45:09Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66263
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University