BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF LIMA BEAN YELLOW MOSAIC VIRUS
KrishiKosh
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Title |
BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF LIMA BEAN YELLOW MOSAIC VIRUS
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Creator |
M, BHAGYASHREE
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Contributor |
H A, PRAMEELA
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Subject |
beans, dna, vegetables, diseases, biological phenomena, viruses, genetic structures, planting, proteins, pcr
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Description |
Yellow mosaic disease caused by a Begomovirus is an important production constraint in lima bean cultivation in Karnataka. Survey conducted during 2013-14 to assess the yellow mosaic disease incidence in lima bean growing areas of selected districts of southern dry zone of Karnataka revealed the occurrence of the disease in the range of 25.3 to 81.1 per cent. Among the districts surveyed, highest average disease incidence was recorded in Chikkaballapura (72.03%) and least incidence was recorded in Ramanagara (31.95%) district. Lima bean yellow mosaic virus (LYMV) was successfully transmitted to French bean, horsegram, pole bean, ring bean and soybean through whiteflies (Bemisia tabaci). The virus was detected by PCR using viral coat protein (CP) and movement protein (MP) specific primers, which amplified ~1000bp and ~900bp product corresponding to the CP and MP gene of the virus. The sequence of the CP and MP shared maximum nucleotide identity of 98% and 99% with Horsegram yellow mosaic virus (HgYMV) isolates infecting French bean (FB) and horsegram from Bangalore. The DNA-A component (2.7kb) of the virus was also amplified and sequenced. The sequences showed maximum nucleotide identity of 99% with HgYMVBangalore: FB isolate. Phylogenetic analysis of the sequences of LYMV genomic components viz., DNA-A, MP and CP genes revealed that LYMV-GKVK isolate clustered with HgYMV infecting horsegram and French bean isolates of Tamil Nadu, Srilanka and Bangalore, which indicates that the virus causing lima bean yellow mosaic disease is a strain of HgYMV. |
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Date |
2016-10-25T12:59:01Z
2016-10-25T12:59:01Z 2014-07-08 |
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Type |
Thesis
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Identifier |
TH-10773
http://krishikosh.egranth.ac.in/handle/1/81796 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences GKVK, Bangalore
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