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ASSESSMENT OF GENETIC DIVERGENCE OF Zingiber officinale ROSC. IN HIMACHAL PRADESH USING MOLECULAR MARKERS

KrishiKosh

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Title ASSESSMENT OF GENETIC DIVERGENCE OF Zingiber officinale ROSC. IN HIMACHAL PRADESH USING MOLECULAR MARKERS
 
Creator PAWAR, KIRAN RAMESH
 
Contributor SHIRKOT, POONAM
 
Subject livestock, diseases, viruses, biological phenomena, pcr, sampling, electrophoresis, biotechnology, genes, dna
Zingiber officinale Rosc,genetic diversity,morphological and isozymic markers
 
Description ABSTRACT
Zingiber officinale Rosc. Commonly known as ginger is a very important spice and
medicinal crop. Ginger belongs to the family Zingiberaceae. In the present investigation an
attempt has been made to assess the genetic diversity of Zingiber officinale Rosc. using
morphological and isozymic markers. Four qualitative and nine quantitative characters have been
studied but only quantitative morphological descriptors showed significant variation. The isozyme
analysis was conducted for eight enzyme systems and all of them showed significant activity
producing typical banding patterns. The five markers viz. MDH Ia, PER Ia, PER Ib, PER IIIa,
EST IIa. at positions of RM values 0.78, 0.82, 0.68, 0.16 and 0.33 respectively produced
significant polymorphism and successfully demarcated the four sites of Sirmaur and Shimla
districts from four sites of Bilaspur and Hamirpur districts. However the five enzyme systems
alkaline phosphatase, aspartate amino transferase, catalase, alcohol dehydrogenase and
phosphorylase were found to be monomorphic in nature. According to dendrogram constructed
using UPGMA and NTSYS-pc version 2.0, the twenty four genotypes of eight sites of four
districts were bifurcated, differentiating the twenty four genotypes from four districts of Himachal
Pradesh into two major clusters. The upper cluster demarcated the four sites from districts
Sirmaur & Shimla of upper Himachal Pradesh with the altitude range 932-1400m whereas the
lower cluster consisted of four sites from the comparatively lower altitude (350-1100m) districts
of Bilasur and Hamirpur. Allele frequency in present study ranged from 0.5-1.0. Polymorphism in
the selected twenty four genotypes of Zingiber officinale Rosc. was found to be 18.51 per cent,
depicting low variability.
 
Date 2017-01-03T15:10:38Z
2017-01-03T15:10:38Z
2011
 
Type Thesis
 
Identifier 47696
http://krishikosh.egranth.ac.in/handle/1/94196
 
Language en
 
Format application/pdf