ICAR Research Data Repository for Knowledge Management
Cloning of Erns Gene of Classical Swine Fever Virus and Characterization of the Expressed Recombinant Protein in Prokaryotic System
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Cloning of Erns Gene of Classical Swine Fever Virus and Characterization of the Expressed Recombinant Protein in Prokaryotic System
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| Creator |
Raviprasadh, R.
Manoharan, S. Kumanan, K. |
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| Description |
Classical swine fever virus Erns gene (681 bp) was cloned into pET22b vector using DH5 a cells and further expression was done by transforming into BL21 (DE3) Codon Plus RIL cells. The recombinant protein expression was conīrmed by SDS-PAGE and western blotting methods using both monoclonal and polyclonal antibodies in dimeric form under non-denaturing condition retaining the conformational epitopes. This could be used as a coating antigen for developing discriminatory diagnostic ELISAs for regular sero-monitoring of animals and also during outbreak situations.
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| Date |
2016-08-08T12:51:03Z
2016-08-08T12:51:03Z 2013-10 |
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| Type |
Article
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/71496
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| Language |
en
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| Format |
application/pdf
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| Publisher |
Indian Vet. J.
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