DEVELOPMENT OF TRANSGENIC POMEGRANATE Cv BHAGWA FOR BACTERIAL BLIGHT RESISTANCE
KrishiKosh
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Title |
DEVELOPMENT OF TRANSGENIC POMEGRANATE Cv BHAGWA FOR BACTERIAL BLIGHT RESISTANCE
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Creator |
SWETHA, H G
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Contributor |
SUKHADA, MOHANDAS
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Subject |
planting, pomegranates, regeneration, diseases, genetic processes, bacteria, genes, biological phenomena, vegetative propagation, concentrates
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Description |
Pomegranate is an economically important species of the tropical and subtropical regions of the world, due to its delicious edible fruits, pharmaceutical and ornamental usage. Pomegranate cultivation and production are threatened by many pests and diseases. By far the most serious disease is bacterial blight which is caused by the air borne bacteria Xanthomonas axanopodis pv.punicae . It is a serious disease, causing devastation in the pomegranate growing areas. In the present study transgenic pomegranate plants of variety Bhagwa developed by transformation with the antimicrobial peptide (AMP) gene cloned from onion seeds and with constructs developed in pCAMBIA 2301. Explants like nodes, leaf, petals and cotyledons from polyhouse were micropropagated in different combination of hormones and the invitro plantlets were co-cultivated with Agrobacterium with binary vector pCAMBIA 2301 having nptII (Neomycin phosphotransferase-II) as a selectable marker gene and AMP as a resistant gene. Transformants were rigorously selected on the selection medium (kanamycin 100 mg/l). The integration of AMP gene was confirmed by PCR. Out of 4 putative transformants 3 transformants confirmed the integration of AMP gene by PCR. In nodes, out of 2 putative transformants 1 showed amplification of AMP gene by PCR analysis. Therefore, the method standardized in the present study can be successfully used for transformation of Pomegranate. |
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Date |
2016-12-15T10:16:30Z
2016-12-15T10:16:30Z 2011-03-26 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/90244
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences GKVK, Bangalore
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