Record Details

DEVELOPMENT OF TRANSGENIC POMEGRANATE Cv BHAGWA FOR BACTERIAL BLIGHT RESISTANCE

KrishiKosh

View Archive Info
 
 
Field Value
 
Title DEVELOPMENT OF TRANSGENIC POMEGRANATE Cv BHAGWA FOR BACTERIAL BLIGHT RESISTANCE
 
Creator SWETHA, H G
 
Contributor SUKHADA, MOHANDAS
 
Subject planting, pomegranates, regeneration, diseases, genetic processes, bacteria, genes, biological phenomena, vegetative propagation, concentrates
 
Description Pomegranate is an economically important species of the tropical and
subtropical regions of the world, due to its delicious edible fruits, pharmaceutical
and ornamental usage. Pomegranate cultivation and production are threatened by
many pests and diseases. By far the most serious disease is bacterial blight
which is caused by the air borne bacteria Xanthomonas axanopodis pv.punicae .
It is a serious disease, causing devastation in the pomegranate growing areas. In
the present study transgenic pomegranate plants of variety Bhagwa developed by
transformation with the antimicrobial peptide (AMP) gene cloned from onion
seeds and with constructs developed in pCAMBIA 2301. Explants like nodes,
leaf, petals and cotyledons from polyhouse were micropropagated in different
combination of hormones and the invitro plantlets were co-cultivated with
Agrobacterium with binary vector pCAMBIA 2301 having nptII (Neomycin
phosphotransferase-II) as a selectable marker gene and AMP as a resistant gene.
Transformants were rigorously selected on the selection medium (kanamycin
100 mg/l). The integration of AMP gene was confirmed by PCR. Out of 4
putative transformants 3 transformants confirmed the integration of AMP gene
by PCR. In nodes, out of 2 putative transformants 1 showed amplification of
AMP gene by PCR analysis. Therefore, the method standardized in the present
study can be successfully used for transformation of Pomegranate.
 
Date 2016-12-15T10:16:30Z
2016-12-15T10:16:30Z
2011-03-26
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/90244
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore