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Physiological phenotyping of soybean (Glycine max L. Merrill) genotypes for seed longevity

KrishiKosh

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Title Physiological phenotyping of soybean (Glycine max L. Merrill) genotypes for seed longevity
M.Sc.
 
Creator Mohammad Zahid
 
Contributor SK Jain
 
Subject sowing, genotypes, soybeans, storage, enzymes, germinability, developmental stages, yields, seed treatment, land resources
 
Description “Physiological phenotyping of soybean genotypes for seed longevity”
26 soybean genotypes, comprising 13 black-seeded-good storer and 13 yellow-seededpoor
storer and another set of 15 genotypes comprising 11 advance breeding lines, 2 each
from good and 2 poor storer genotypes were selected on the basis of their reported
storability. Seeds of 26 genotypes were grown in one cycle of multiplication to bring
uniform physiological status of seeds and were stored for a period of 24 and 36 months at
controlled environment (8°C and 35% RH). Seeds retrieved from storage and conditioned
to lab temperature before use. From set of 15 genotypes stored at Delhi ambient storage
conditions for seven months, crop was raised, on 75 cm apart raised ridges in CRBD.
From this fresh produce, seeds were used as reference material. These were subjected to
physical, physiological, and biochemical phenotyping.
Genotypic variability with respect to seed storability was observed among different
soybean genotypes. Black-seeded small soybean genotypes, showed better storability. A
significant reduction in seed coat hardiness was observed with increase in storage period;
however the reduction was much more under ambient seed storage than those of controlled
storage.
Seed germination, AA, RSG, seedling growth, seed vigour index I & II,
irrespective of genotypes, decreased with increased period of seed storage, whereas EC,
gradually increased with increase storage period. Under Delhi ambient storage
environment, cream-/yellow-seeded poor storer genotypes registered marginal increase in
100-seed weight with increase storage period, whereas it decreased under controlled
storage environment. Volatile aldehydes, esterase enzyme activity increased with increase
period of seed storage, whereas superoxide dismutase activity consistently declined with
the increased period of seed storage
Based on detailed physiological and biochemical phenotyping, four genotypes, namely
DS-12-13, DS-2614, SL-799 and SL-525 were identified as good performers/storer. The
good storability of soybean seeds can be attributed to the combined effect of seed coat
(testa) colour, seed size, seed coat hardiness; free radical mediated chain reactions leading
to bio-membrane permeability, efficiency of antioxidant system in the seed to counter free
radical accumulation.
The seed germination as well seed hardiness were either +vely or –vely but significantly
correlated with all the traits except for the trait of 100-seed weight and/or field emergence.
The data showed a strong +ve correlation between accelerated ageing and seed hardiness,
followed by accelerated ageing and seed germination, whereas a strong but negative
correlation was observed between volatile aldehydes and seed hardiness.
Based on my detailed physical, physiological and biochemical, investigations on freshly
harvested and stored seed; the volatile aldehydes and/or seed hardiness are suggested as
better indices of soybean seed quality. From the above two, any parameter be chosen – as
a good marker for seed quality in soybean.
 
Date 2016-03-18T13:38:22Z
2016-03-18T13:38:22Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65264
 
Language en_US
 
Format application/pdf
 
Publisher IARI, DIVISION OF SEED SCIENCE AND TECHNOLOGY Indian Agricultural Research Institute New Delhi