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STUDIES ON THE DIVERSITY IN MORPHOLOGICAL, BIO-CHEMICAL ANDMOLECULAR CHARACTERIZATION IN SAPOTA (Manilkaraachras (Mill.) Fosberg) GENOTYPES

KrishiKosh

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Title STUDIES ON THE DIVERSITY IN MORPHOLOGICAL, BIO-CHEMICAL ANDMOLECULAR CHARACTERIZATION IN SAPOTA (Manilkaraachras (Mill.) Fosberg) GENOTYPES
 
Creator A. HARSHAVARDHAN
 
Contributor Dr. M.Lakshminarayana Reddy
 
Subject diversity in morphological, bio-chemical and molecular characterization,tree morphological, fruit bio-chemical and molecular markers, quantitative traits , phenotypic coefficient of variation, genotypic coefficient of variation, heritability and genetic advance , Correlation coefficient and Path coefficient analysis ,D2 analysis,RAPD analysis ,
 
Description The present investigation, “Studies on the diversity in morphological, bio-chemical and molecular characterization in sapota genotypes”, was carried out during 2013-2014 at Horticultural Research Station, Venkataramannagudem, Andhra Pradesh. Genetic diversity was evaluated in thirty three genotypes of sapotaby tree morphological, fruit bio-chemical and molecular markers. Twenty four RAPD and sixteen SSR markers were employed to analyze the molecular diversity among the genotypes.
The analysis of variance for the twenty three quantitative traits revealed significant differences for all the characters studied thus indicating wide variation among the genotypes.The genotype Kirthibarthi recorded maximum plant height, while the genotype Cricket Ball recorded maximum leaf length, leaf width, leaf area and seed weight. The mean fruit length was highest in PKM-4 and maximum fruit width, fruit weight and pulp to seed ratio was recorded in Columbian Sapota. The genotype Tagarampudi recorded highest per cent fruit set and yield/tree. Further, the genotype PKM-4 recorded maximum TSS and total sugars, while maximum reducing sugars was reported in Kalipatti and highest non-reducing sugars was recorded in the genotype CO-2. Highest amount of ascorbic acid content was recorded in CO-1, while maximum titrable acidity and pectin was reported in Mirandi. Further, the genotypeKrishna Rao recorded highest total phenols.
The characters, namely, fruit weight, seed number, seed weight, pulp to seed ratio, per cent fruit set, yield per tree, non-reducing sugars, ascorbic acid, TSS to acid ratio and pectins had recorded higher estimates for phenotypic coefficient of variation, genotypic coefficient of variation, heritability and genetic advance as per cent mean.
Correlation coefficient and Path coefficient analysis revealed that the association of plant height, leaf width, leaf area, fruit width, fruit weight, seed weight, pulp to seed ratio and per cent fruit set with yield/tree and among themselves was positive and highly significant and these traits were identified as fruit yield components and exerted high positive direct influence on yield per tree. Similarly the association of TSS, total sugars, reducing sugars, non-reducing sugars, ascorbic acid and TSS to Acid ratio with yield per tree among themselves was positive and highly significant. This indicated that direct selection of yield improvement through these traits would be rewarding.
In D2 analysis, the characters viz., leaf width, leaf area, fruit length, per cent fruit set, TSS, total sugars, non-reducing sugars, ascorbic acid and TSS to Acid ratio contributed more for the divergence. In PCA, the characters viz., yield per tree, total sugars, TSS, ascorbic acid, titrable acidity, TSS to Acid ratio, phenols and per cent fruit set in PC1 contributing more towards variability.
The RAPD analysis with twenty four primers produced 204 polymorphic bands with an average of 8.2 polymorphic bands per primer. Out of total 204 polymorphic bands, thirteen bands were unique to particular genotype viz., Gutti (OPC-2), Tagarampudi and PKM-4 (OPG-3), Calcutta Round, Columbian Sapota and Cricket Ball (OPG-4), Pakala Oval (OPG-7), DHS-2 (OPV-9), DHS-1 (OPV-10), Columbian Sapota (OPX-3), Cricket Ball (OPX-11), Kalipatti and Pakala Round (OPX-12) which could be exploited for DNA fingerprinting of these accessions by converting RAPD markers into STS markers and this was highly useful for detecting mixes between genotypes.
Eighty eight alleles were produced by examining the 33 sapota genotypes with sixteen microsatellite loci with an average number of 2.66 alleles per locus. High level of polymorphism was observed with primers SSR S-4 and SSR Mh-12. The PCR product size obtaind by amplification of SSR primers was ranged from 135 to 555 bp. Out of the total 88 polymorphic alleles, twenty one fragments were unique to particular genotypes viz., Columbian Sapota, DHS-1 and DHS-2 (SSR S-1), CO-3 (SSR S-2), Pakala Round (SSR S-3), Seedless (SSR S-4), PKM-2 (SSR S-5), DHS-1 and DHS-2 (SSR S-7), Cricket Ball, Calcutta Round and CO-2 (SSR S-8), Gavarayya (SSR S-9), Singapore, DHS-1 and PKM-2 (SSR S-10), CO-2 (SSR Mh-26), Bombay (SSR Mh-20), Kalipatti and Pakala Round (SSR Mh-12) and Pala (SSR Mh-17) which could be exploited for DNA fingerprinting of these genotypes.Based on outcome of the present investigation, it can be concluded that molecular markers for fingerprinting as well as estimation of genetic diversity and genetic relatedness in sapota genotypes is effective, precise and more efficient than morphological markers.
 
Date 2016-07-28T14:26:18Z
2016-07-28T14:26:18Z
2015-08
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/70175
 
Language en
 
Format application/pdf
 
Publisher Dr.Y.S.R. Horticultural Univeristy