STUDY OF BC1 PROGENY IN Bt TRANSGENIC SORGHUM PLANTS WITH Cry1B GENE
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Title |
STUDY OF BC1 PROGENY IN Bt TRANSGENIC SORGHUM PLANTS WITH Cry1B GENE
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Creator |
SRUJANA, S
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Contributor |
Bharathi, M
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Subject |
STUDY, BC1 PROGENY, Bt, TRANSGENIC, SORGHUM, PLANTS, Cry1B, GENE
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Description |
The present investigation was carried out during rabi, 2005 to Summer, 2006 in sorghum (Sorghum bicolor (L.) Moench) at Biotechnology Unit, at National Research Centre for Sorghum, Hyderabad. Agronomically superior transgenic sorghum plants resistant to stem borer were produced by backcrossing of transgenic sorghum plants with non-transformed parental lines and they were evaluated for the expression of Cry1B gene through PCR and ELISA techniques. Initially, transformed and non-transformed lines of genotype CS3541 were characterized for morphological characters, followed by backcrossing transgenic plant with non-transformed CS3541. Parallelly, a hybrid cross to male sterile 296A (a popular seed parent used for production of hybrids) was done to verify the transgene expression in hybrid progeny. Among the twelve morphological characters studied, transgenic progeny in T1 and T2 generations varied significantly with non-transformed lines for characters, days to 50% flowering, stem girth, panicle width, total seed weight, number of primary branches and pollen fertility in all the transgenic events. All these characters except days to 50% flowering in transgenic progeny were significantly lower than that of non-transformed lines. Transgenic progeny were significantly taller than non-transformed lines in all transgenic events except in the event 24 of T1 generation. They had significantly less number of leaves than non-transformed lines in all transgenic events except in the event 14 of T1 generation. They produced seed of low 100 seed weight in all transgenic events except in the event 24 of T2 generation. They had significantly shorter panicles in T1 generation while the T2 generation transgenic progeny plants were not significantly different in panicle length. In the case of leaf breadth and leaf length there was no significant difference between transformed and non-transformed plants. On the whole, variation in T2 generation was less than that in T1 generation, although the variation at T2 generation was more than what we expected. Thus, there was need for one or two backcrosses to improve these characters and develop isogenic lines with Cry1B gene. Integration of the transgene in backcross progeny and hybrid plants was verified by PCR. Expression of the transgene was analyzed by ELISA in confirmed progeny plants. Cry1B expression in hybrid plants was relatively more than their respective backcross progeny. Since sorghum has tended to be the most recalcitrant crop for tissue culture, regeneration and genetic transformation, the application of transgenic approaches to genetic improvement of sorghum has lagged compared to other cereal crops. In planta transformation proves to be promising technology for producing more transgenic plants. The putative in planta transformants were evaluated through PCR analysis |
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Date |
2016-08-16T13:34:39Z
2016-08-16T13:34:39Z 2006 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/72556
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Language |
en
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Relation |
D7815;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD
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