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STUDY OF BC1 PROGENY IN Bt TRANSGENIC SORGHUM PLANTS WITH Cry1B GENE

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Title STUDY OF BC1 PROGENY IN Bt TRANSGENIC SORGHUM PLANTS WITH Cry1B GENE
 
Creator SRUJANA, S
 
Contributor Bharathi, M
 
Subject STUDY, BC1 PROGENY, Bt, TRANSGENIC, SORGHUM, PLANTS, Cry1B, GENE
 
Description The present investigation was carried out during rabi, 2005 to Summer, 2006 in
sorghum (Sorghum bicolor (L.) Moench) at Biotechnology Unit, at National Research
Centre for Sorghum, Hyderabad. Agronomically superior transgenic sorghum plants
resistant to stem borer were produced by backcrossing of transgenic sorghum plants with
non-transformed parental lines and they were evaluated for the expression of Cry1B gene
through PCR and ELISA techniques. Initially, transformed and non-transformed lines of
genotype CS3541 were characterized for morphological characters, followed by
backcrossing transgenic plant with non-transformed CS3541. Parallelly, a hybrid cross to
male sterile 296A (a popular seed parent used for production of hybrids) was done to
verify the transgene expression in hybrid progeny.
Among the twelve morphological characters studied, transgenic progeny in T1 and
T2 generations varied significantly with non-transformed lines for characters, days to 50%
flowering, stem girth, panicle width, total seed weight, number of primary branches and
pollen fertility in all the transgenic events. All these characters except days to 50%
flowering in transgenic progeny were significantly lower than that of non-transformed
lines. Transgenic progeny were significantly taller than non-transformed lines in all
transgenic events except in the event 24 of T1 generation. They had significantly less
number of leaves than non-transformed lines in all transgenic events except in the event
14 of T1 generation. They produced seed of low 100 seed weight in all transgenic events
except in the event 24 of T2 generation. They had significantly shorter panicles in T1
generation while the T2 generation transgenic progeny plants were not significantly
different in panicle length. In the case of leaf breadth and leaf length there was no
significant difference between transformed and non-transformed plants. On the whole,
variation in T2 generation was less than that in T1 generation, although the variation at T2
generation was more than what we expected. Thus, there was need for one or two
backcrosses to improve these characters and develop isogenic lines with Cry1B gene.
Integration of the transgene in backcross progeny and hybrid plants was verified
by PCR. Expression of the transgene was analyzed by ELISA in confirmed progeny
plants. Cry1B expression in hybrid plants was relatively more than their respective
backcross progeny. Since sorghum has tended to be the most recalcitrant crop for tissue
culture, regeneration and genetic transformation, the application of transgenic approaches
to genetic improvement of sorghum has lagged compared to other cereal crops. In planta
transformation proves to be promising technology for producing more transgenic plants.
The putative in planta transformants were evaluated through PCR analysis
 
Date 2016-08-16T13:34:39Z
2016-08-16T13:34:39Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72556
 
Language en
 
Relation D7815;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD