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IN-SILICO AND IN-VITRO EVALUATION OF DRUGGABILITY PRIORITIZATION TARGETS OF E. COLI IN COMMERCIAL BROILERS

KrishiKosh

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Title IN-SILICO AND IN-VITRO EVALUATION OF DRUGGABILITY PRIORITIZATION TARGETS OF E. COLI IN COMMERCIAL BROILERS
 
Creator K. USHA RANI
 
Contributor Dr. A. GOPALA REDDY
 
Subject sowing, genetics, developmental stages, biological development, biological phenomena, vegetative propagation, selection, germinability, rapd, plant habit
 
Description An experimental study was conducted to evaluate the druggability prioritization targets of E.coli in commercial broilers in two experimental phases. In phase I of the experiment, total proteome of pathogen Vs host comparison was done using comparative genomics followed by protein docking of the target proteins of the pathogen and drug target identification and then calculation of drug prioritization parameters for therapeutic targets. Pharmacological evaluation of in vitro antibiogram test of E.coli was done in phase II of experiment. 4,288 proteins of E.coli were compared with 24068 proteins of Gallus gallus using BLASTP analysis. The docking results showed positive ligand poses for proteins 1s7d posed with ligands DB1086, 1053, 484, 636, 1080 and 417, 1sz2 posed with ligands 1053, 1393, 484, 291, 1050, 417, 1291, 1124 and 525, protein 2azo posed with ligands 276, 1086, 1053, 1393, 484,
291, 1050, 47, 2911, 259, 1124, 525 and 316, Protein 2f1f posed with ligand 259, Protein 2glt posed with ligands 1086, 484, 259 and 316, protein 2yva posed with ligands 276, 1086, 1393, 84, 291 and 1050, protein 3hi2 posed with ligands 1086, 484, 100, 259 and 525, protein 1dkl posed with ligands 276, 1393, 1124 and 525, protein 1pho posed with ligands 276, 1086, 1053, 1393, 484, 29, 1050, 417, 291, 259, 1124,525 and 316.
The antibiogram study in phase II revealed the MIC values of all commonly used veterinary antimicrobials along with novel non-antimicrobial lead molecules (based on in silico studies) was performed and it was found that cefpodoxime and moxifloxacin showed the moderate in vitro activity, whereas the lead molecules have not been found with any susceptibilities at the given concentrations. The results of the study revealed further scope for development of potential drug targets for developing novel molecules against E.coli along with the status of susceptibility to different antibiotics.
 
Date 2016-12-27T11:45:32Z
2016-12-27T11:45:32Z
2016-10-31
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/93141
 
Relation D;499
 
Format application/pdf
 
Publisher PVNR TVU