DNA marker assisted multiple trait QTL pyramiding for drought tolerant traits in rice (Oryza sativa L.)
KrishiKosh
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Title |
DNA marker assisted multiple trait QTL pyramiding for drought tolerant traits in rice (Oryza sativa L.)
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Creator |
Haradari, Chandrashekhar
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Contributor |
Shailaja, Hittalamani
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Subject |
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Description |
Rice breeding has led to green revolution in India. Yields of rice has now stabilized or declined due to climate change factors leading to abiotic and biotic stresses. This study was planned to breed for tolerance to abiotic and biotic stress through DNA marker assisted selection. Pyramid genotypes containing genes with blast and bacterial leaf blight were crossed with genotypes containing QTL pyramided for root traits and water use efficiency. Pyramid genotypes were confirmed with SSR markers linked to gene/QTL traits viz., blast resistance gene Pi1 on chromosome 11 with RM144, bacterial blight resistance genes Xa4 on chromosome 11 with RM224 and xa5 on chromosome 5 with RM13, QTL for root traits (viz., qRT2 (RM221-RM318), qRT7 (RM248-RM234), qRT9 (RM242-RM201) on chromosome 2,7,9 respectively) and water use efficiency QTL qWUE2 on chromosome 2 with RM263. Progenies confirmed by SSR markers were evaluated for yield traits in well watered and stress condition and in hot spots. Segregating plants were tiller separated for different evaluation studies. Forty six F2 plants of cross RB6 × QRT25 both pyramided lines were selected and forwarded. F2:3 families of cross RB6 × QRT25 were evaluated for yield component traits in field and root morphological traits in PVC pipes under contrasting moisture regimes by inducing moisture stress for 15 days interval at two crop growth stages under rain-out shelter. Significant differences between pyramided progeny families for selected traits were observed. Among these pyramided genotypes, 23-5-26- qRT2+qRT7+qWUE2+Xa4+xa5 for water use efficiency, 23-5-274-qRT2+qRT7+qRT9+ qWUE2+Pi1+xa5 for blast resistance, 23-5-2-qRT7+qRT9+qWUE2+Pi1+Xa4 for root length, 23-5-277-qRT2+qRT9+qWUE2+Pi-1+Xa4 for grain yield with early flowering, early maturity, high per day yield and high drought resistance index were found superior under moisture stress environments over well water condition. Results showed no yield penalty in pyramid genotypes with genes/QTL conferring to abiotic stress and biotic stress indicating no epistatic interaction. |
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Date |
2016-05-23T11:23:55Z
2016-05-23T11:23:55Z 2013-10-09 |
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Type |
Thesis
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Identifier |
Th-10673
http://krishikosh.egranth.ac.in/handle/1/66204 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences, Bengaluru
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