Record Details

DNA marker assisted multiple trait QTL pyramiding for drought tolerant traits in rice (Oryza sativa L.)

KrishiKosh

View Archive Info
 
 
Field Value
 
Title DNA marker assisted multiple trait QTL pyramiding for drought tolerant traits in rice (Oryza sativa L.)
 
Creator Haradari, Chandrashekhar
 
Contributor Shailaja, Hittalamani
 
Subject ---
 
Description Rice breeding has led to green revolution in India. Yields of rice has now stabilized or
declined due to climate change factors leading to abiotic and biotic stresses. This study was
planned to breed for tolerance to abiotic and biotic stress through DNA marker assisted selection.
Pyramid genotypes containing genes with blast and bacterial leaf blight were crossed with
genotypes containing QTL pyramided for root traits and water use efficiency. Pyramid genotypes
were confirmed with SSR markers linked to gene/QTL traits viz., blast resistance gene Pi1 on
chromosome 11 with RM144, bacterial blight resistance genes Xa4 on chromosome 11 with
RM224 and xa5 on chromosome 5 with RM13, QTL for root traits (viz., qRT2 (RM221-RM318),
qRT7 (RM248-RM234), qRT9 (RM242-RM201) on chromosome 2,7,9 respectively) and water
use efficiency QTL qWUE2 on chromosome 2 with RM263. Progenies confirmed by SSR
markers were evaluated for yield traits in well watered and stress condition and in hot spots.
Segregating plants were tiller separated for different evaluation studies. Forty six F2 plants of
cross RB6 × QRT25 both pyramided lines were selected and forwarded. F2:3 families of cross
RB6 × QRT25 were evaluated for yield component traits in field and root morphological traits in
PVC pipes under contrasting moisture regimes by inducing moisture stress for 15 days interval at
two crop growth stages under rain-out shelter. Significant differences between pyramided
progeny families for selected traits were observed. Among these pyramided genotypes, 23-5-26-
qRT2+qRT7+qWUE2+Xa4+xa5 for water use efficiency, 23-5-274-qRT2+qRT7+qRT9+
qWUE2+Pi1+xa5 for blast resistance, 23-5-2-qRT7+qRT9+qWUE2+Pi1+Xa4 for root length,
23-5-277-qRT2+qRT9+qWUE2+Pi-1+Xa4 for grain yield with early flowering, early maturity,
high per day yield and high drought resistance index were found superior under moisture stress
environments over well water condition. Results showed no yield penalty in pyramid genotypes
with genes/QTL conferring to abiotic stress and biotic stress indicating no epistatic interaction.
 
Date 2016-05-23T11:23:55Z
2016-05-23T11:23:55Z
2013-10-09
 
Type Thesis
 
Identifier Th-10673
http://krishikosh.egranth.ac.in/handle/1/66204
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru