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Computational and Expression Analysis of Anthocyanin Biosynthesis Genes in Tomato (Solanum lycopersicum L.)

KrishiKosh

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Title Computational and Expression Analysis of Anthocyanin Biosynthesis Genes in Tomato (Solanum lycopersicum L.)
 
Creator DINABANDHU BEHERA
 
Contributor T. R. Sharma
 
Subject genes, anthocyanins, vegetables, planting, fruits, aromatic compounds, enzymes, proteins, genomes, pcr
 
Description T-8585
Anthocyanins provide diverse kinds of functions in plants and offer huge human health
benefits. The latest tomato genome draft sequence version release 2.40 was used to
analyze anthocyanin genes, annotate gene structures and to determine their exact
physical locations in different chromosomes. The position of 5′ UTR, 3′ UTR, length of
ORF and upstream basal promoters were detected for seven anthocyanin pathway genes
viz. CHS, CHI, F3H, F3′5′H, DFR, ANS, and UF3GT. The genes F3H, DFR, ANS, and
UF3GT were of single copy each whereas CHS, CHI, and F3′5′H were having two
copies each. Physically the genes CHI, F3H, F3′5′H, DFR, ANS, and UF3GT were
located in chromosomes 5, 2, 11, 2, 8 and 10, respectively. The two genes CHS1 and
CHS2 have been identified in chromosomes 5 and 8 respectively. Except CHI, all the
genes were present on long arm of chromosomes. The 1kb upstream region was
analyzed by PLACE database to find cis regulatory elements (CRE) motifs. Out of 145
types of CREs found in the seven genes, 93 have been annotated to involve in plant
stress responses and 16 in binding sites for MYB and bHLH transcription factors
regulating the expression of seven genes for anthocyanin production. Expression analysis
of these genes carried out by both RT- PCR and RT-qPCR showed that CHI, DFR and
ANS might be the rate limiting enzymes for non-production of anthocyanin in the
cultivated tomatoes. Comparative analysis of six common anthocyanin pathway genes
between different species of solanaceae and poaceae showed that the downstream
enzymes evolve more rapidly than the upstream enzymes and CHI might have
undergone ancient duplication and subsequent divergence during evolution. Predicted
protein structures between tomato and maize revealed that CHS was highly conserved
whereas CHI was highly variable. Expression analyses of anthocyanin biosynthesis
genes of Indian tomato cultivars in present investigation will act as important basis for
understanding of the development of anthocyanin enriched tomatoes since the fruit is
consumed as a favorite dish.
 
Date 2016-12-15T10:58:26Z
2016-12-15T10:58:26Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/90267
 
Format application/pdf
 
Publisher IARI, DIVISION OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY