GENETIC CHARACTERIZATION OF TOMATO (Lycopersicon esculentum Mill.) GENOTYPES USING RAPD
KrishiKosh
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Title |
GENETIC CHARACTERIZATION OF TOMATO (Lycopersicon esculentum Mill.) GENOTYPES USING RAPD
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Creator |
ROSY
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Contributor |
SRIVASTAVA, D.K.
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Subject |
guavas, productivity, fruits, planting, crops, area, land resources, irrigation, orchards, tillage equipment
tomato (Lycopersicon esculentum Mill.) genotypes using RAPD |
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Description |
Abstract The present studies on ‘‘Genetic characterization of tomato (Lycopersicon esculentum Mill.) genotypes using RAPD” was carried out to study the genetic relationship among various genotypes. Besides the proposed objective, work on Simple Sequence Repeats (SSR) was also done. The aim of this study was to investigate genetic relationship among different genotypes of tomato. 21 tomato genotypes namely 1-(EC- 28373), 2-(Pepsi92), 3-(SolanVajr), 4-(EC-521086), 5-(EC-528374), 6-(101), 7-(UHF- 517), 8-(UHF-562), 9-(UHF-659), 10-(UHF-521), 11-(EC-528367), 12-(AC-402), 13- (110264), 14-(AL-14), 15-(UHF-562), 16-(UHF-506), 17-(UHF-571), 18-(UHF-505), 19- (UHF-55), 20-(UHF-571), 21-(EC-521074) were selected for the study. A total of 25 primers were tried to generate RAPD profile, out of these reproducible patterns were obtained with 15 primers. A total of 72 bands were obtained out of which all were polymorphic and 3 were unique bands and in case of SSR studies 9 primers were used and total number of bands obtained were 33 with 3 unique bands. The percentage of total polymorphism was 100% in both RAPD and SSR. Similarity index was computed based on Jacquard coefficient and used for cluster analysis based on UPGMA. RAPD and SSR technology could be useful for identification of different accessions as well as accessing the genetic similarity among different accessions of tomato. |
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Date |
2017-01-03T14:51:53Z
2017-01-03T14:51:53Z 2012 |
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Type |
Thesis
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Identifier |
47701
http://krishikosh.egranth.ac.in/handle/1/94178 |
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Language |
en
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Format |
application/pdf
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