STUDIES ON GENETIC FIDELITY IN MICROPROPAGATED SHOOTS OF APPLE ROOTSTOCK MERTON I. 793 USING DNA MARKERS
KrishiKosh
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Title |
STUDIES ON GENETIC FIDELITY IN MICROPROPAGATED SHOOTS OF APPLE ROOTSTOCK MERTON I. 793 USING DNA MARKERS
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Creator |
CHOUDHARY, SHRUTI
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Contributor |
MODGIL, MANJU
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Subject |
diseases, enzymes, sexual reproduction, organic compounds, biological phenomena, fruits, breeds (animals), byproducts, sampling, potassium
Random amplified polymorphic DNA (RAPDs),simple sequence repeats (SSR), |
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Description |
ABSTRACT Random amplified polymorphic DNA (RAPDs) and simple sequence repeats (SSR) were used to evaluate the clonal stability of four year old micropropagated shoot clusters of Merton I.793. Shoots multiplied under in vitro conditions on suitable medium were categorized on the basis of three morphotypes with minor differences. For assessment of genetic fidelity, DNA was isolated from leaves of mother plant grown in the field and in vitro raised shoot cultures of MI.793. Samples with good quality DNA were selected for amplification reaction. Out of the 31 RAPD primers screened, 27 produced a total of 130 scorable and distinct bands with an average of 3.65 bands per primer. 26 primers resulted in monomorphism across all the shoot clusters while, little (2.3%) polymorphisim was obtained with a single primer where 3 bands were absent in four shoot clusters. Of the 6 SSR primers, 5 generated 9 bands with an average of 1.8 bands per primer indicating complete monomorphism. The banding patterns for each primer were highly uniform and comparable to the mother clone from which the cultures had been established. DNA markers revealed genetic similarities within micropropagated shoots and mother plant of Merton I. 793. Our results show that micropropagation protocol for this rootstock is reliable and emphasizes its utility for large scale propagation over a long period of time, without much risk of genetic instability. |
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Date |
2017-01-03T11:55:54Z
2017-01-03T11:55:54Z 2012 |
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Type |
Thesis
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Identifier |
47730
http://krishikosh.egranth.ac.in/handle/1/94116 |
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Language |
en
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Format |
application/pdf
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