Development of efficient protocol for micro-propagation of potato (Solanum tuberosum L.)
KrishiKosh
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Title |
Development of efficient protocol for micro-propagation of potato (Solanum tuberosum L.)
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Creator |
Mohapatra, Priyadarshani P.
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Contributor |
Batra, V.K.
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Subject |
True-to-type, In vitro, Solanum tuberosum, Plant Growth regulators
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Description |
Solanum tuberosum L. is one of the world s most economically important tuber crops and belongs to family Solanaceae. It is the fourth most cultivated food crop in the world after wheat, rice and maize. Potato produces more edible energy/proteins per unit area as compare to other crops and is major staple food in many countries. Conventionally, plants are propagated through tubers. Due to low multiplication rate and high susceptibility to various diseases, there is a need to develop an alternate method of propagation. Therefore, Plant Tissue Culture technique can be used for the mass production of true-to-type, disease free quality planting material of potato cultivar Kufri Pukhraj and Kufri Frysona. In the present investigation, experiments were conducted to development of efficient protocol for micro-propagation of potato (Solanum tuberosum L.). Sprouts and Shoot tips were used as explants. Sterilization of explants were done using bavistin (0.2%) and streptocycline (0.4 %) for 45 minutes followed by treatment with HgCl2 (0.1%) for 60 seconds. Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Pukhraj maximum shoots (21.3) and in Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l IBA in Kufri Pukhraj and MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The experiment on microtuberization with nodal segment from well multiplied cultures were taken and kept in media containing MS basal along with different combination and concentration of growth regulators and sugar. The maximum number of microtuber (3.8) were obtained in PTM3 (MS basal salt + 0.01mg/l BAP + 0.01mg/l NAA + 0.1mg/l GA3 + Sugar 80g/l) in 50.1 days with 9.4mm size and 0.4g weight in cv. Kufri Pukhraj whereas in cv. Kufri Frysona maximum number of microtuber (2.5) were obtained on same medium in 52.3 days with maximum 9.0 mm size and 0.27 g weight. The protocol will be very useful for large-scale production of S. tuberosum in future. |
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Date |
2016-09-15T09:21:39Z
2016-09-15T09:21:39Z 2014 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/76787
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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