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Development of efficient protocol for micro-propagation of potato (Solanum tuberosum L.)

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Title Development of efficient protocol for micro-propagation of potato (Solanum tuberosum L.)
 
Creator Mohapatra, Priyadarshani P.
 
Contributor Batra, V.K.
 
Subject True-to-type, In vitro, Solanum tuberosum, Plant Growth regulators
 
Description Solanum tuberosum L. is one of the world s most economically important
tuber crops and belongs to family Solanaceae. It is the fourth most cultivated food crop in the
world after wheat, rice and maize. Potato produces more edible energy/proteins per unit area
as compare to other crops and is major staple food in many countries. Conventionally, plants
are propagated through tubers. Due to low multiplication rate and high susceptibility to
various diseases, there is a need to develop an alternate method of propagation. Therefore,
Plant Tissue Culture technique can be used for the mass production of true-to-type, disease
free quality planting material of potato cultivar Kufri Pukhraj and Kufri Frysona. In the
present investigation, experiments were conducted to development of efficient protocol for
micro-propagation of potato (Solanum tuberosum L.). Sprouts and Shoot tips were used as
explants. Sterilization of explants were done using bavistin (0.2%) and streptocycline (0.4 %)
for 45 minutes followed by treatment with HgCl2 (0.1%) for 60 seconds. Sterilized explants
were inoculated on MS basal supplemented with various growth regulators and established
successfully. The sprouted explants were further sub-cultured on MS media supplemented
with various growth regulator alone and in combination for in vitro multiplication. In Kufri
Pukhraj maximum shoots (21.3) and in Kufri Frysona (11.2) shoots were obtained on MS
medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro
rooting was observed on MS basal medium supplemented with 2.0 mg/l IBA in Kufri Pukhraj
and MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days.
Rooted plantlets were successfully hardened in green house using different types of potting
mixture and finally transferred to field. The experiment on microtuberization with nodal
segment from well multiplied cultures were taken and kept in media containing MS basal
along with different combination and concentration of growth regulators and sugar. The
maximum number of microtuber (3.8) were obtained in PTM3 (MS basal salt + 0.01mg/l BAP
+ 0.01mg/l NAA + 0.1mg/l GA3 + Sugar 80g/l) in 50.1 days with 9.4mm size and 0.4g weight
in cv. Kufri Pukhraj whereas in cv. Kufri Frysona maximum number of microtuber (2.5) were
obtained on same medium in 52.3 days with maximum 9.0 mm size and 0.27 g weight. The
protocol will be very useful for large-scale production of S. tuberosum in future.
 
Date 2016-09-15T09:21:39Z
2016-09-15T09:21:39Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76787
 
Language en
 
Format application/pdf
 
Publisher CCSHAU