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Altered Time Dependent mRNA Expression of GDF-9 And BMP-15 Using Everse Transcriptase-PCR From Buffalo COCs During IVM

KrishiKosh

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Title Altered Time Dependent mRNA Expression of GDF-9 And BMP-15 Using Everse Transcriptase-PCR From Buffalo COCs During IVM
Indian Vet. J., July 2015, 92 (7) : 45 - 48
 
Creator Muralidharan, K.
Eswari, S.
Vijayarani, K.
 
Subject Buffalo oocytes
IVM
Gene expression
GDF-9
BMP - 15
 
Description Growth Differentiation Factor (GDF-9) and Bone Morphogenetic Protein (BMP-15) genes are involved in the regulation of folliculogenesis. This study determines the expression of these two genes during in vitro maturation (IVM) at different time points such as 0 h (immature), 6 h, 12 h and 24 h from buffalo cumulus oocyte complexes using semi-quantitative RT-PCR. The GDF-9 transcripts were detected from cumulus free oocytes during 0 h, 6 h, 12 h and 24 h of in vitro maturation where as in cumulus cells upto 12h of maturation. But the mRNA expression of BMP-15 from cumulus free oocytes was detected during all the four mentioned duration points of IVM, but highly transcribed in immature oocytes and declined during maturation. In case of cumulus cells, BMP-15 transcript was expressed from 0 h upto 12 h and undetectable at 24 h of maturation. This study revealed that both GDF-9 and BMP-15 were expressed in different manner during in vitro maturation of buffalo oocytes.
 
Date 2016-06-07T12:31:02Z
2016-06-07T12:31:02Z
2015-07
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66920
 
Language en
 
Format application/pdf
 
Publisher Indian Veterinary Journal