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Identification of molecular markers for Karnal bunt resistance in wheat (Triticum aestivum L. em. Thell)

KrishiKosh

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Title Identification of molecular markers for Karnal bunt resistance in wheat (Triticum aestivum L. em. Thell)
 
Creator Priyanka
 
Contributor Redhu, A.S.
 
Subject diseases, wheats, grain, developmental stages, genetics, biological phenomena, yields, genotypes, planting, polymorphism
 
Description Present study was carried out to screen 104 recombinant inbred lines (RILs) of cross H 567.71
(resistant) x WH 542 (susceptible) for Karnal bunt resistance and to identify SRAP and RGAP markers
associated with Karnal bunt resistance in wheat. There was significant variation among all the
recombinant inbred lines for percentage and coefficient of infection to Karnal bunt. Most of the RILs
(68) and (71) showed up to 5% infection during the year 2011-12 and 2012-13 and thus were resistant.
The percentage of infected grains or percent infection (PI) in the susceptible RILs was up to 37.14 % in
the year 2011-12 and up to 31.57 % in the year 2012-13 respectively, whereas, range of coefficient of
infection (CI) in the RILs was 0 to 14.21 % in the year 2011-12 and 0 to 13.81 % in the year 2012-13,
respectively. Significant variations were also observed for different morphological traits such as, plant
height (cm), days to flowering, days to maturity, number of tillers/meter length, spike length (cm), no.
of grains/spike, 1000 grain weight, grain yield/meter length (g) and biological yield (g). A total of 80
SRAP and 121 RGAP primer combinations were used to screen parental genotypes. In SRAP, a total of
287 unambiguous bands were amplified by the 20 SRAP of 80 SRAP primer combinations, of which
123 bands were polymorphic (42.85 %). The cluster analysis led to distribution of parents and their
RILs in to two major groups at the similarity coefficient value of 0.55. Major cluster I, included
resistant parent H 567.71 with resistant lines and major cluster II had susceptible parent WH 542 and
susceptible lines, these two groups further subdivided at similarity coefficient of 0.67 into four
different clusters. In RGAP, a total of 89 unambiguous bands were amplified by the 9 of 121 RGAP
primer combinations, of which 46 bands were polymorphic (51.68 %). Genetic similarity by RGAP
analysis showed that parental genotypes were quite distinct from each other. The cluster analysis led to
distribution of parents and their RILs in to two groups at the similarity coefficient value of 0.42. Major
cluster I, included resistant parent H 567.71 with resistant lines and major cluster II had susceptible
parent WH 542 and susceptible lines. These two groups further subdivided into six different clusters at
similarity coefficient of 0.56. By single marker analysis, RGAP 7, RGAP 8 and RGAP 9 were directly
linked with resistance genes which provide resistance against Karnal bunt. Hence, selection of Karnal
bunt resistant genotypes on the basis of these three markers in early generations can be done through
marker assisted selection and could be useful in future development of Karnal bunt resistant varieties.
 
Date 2016-02-09T13:40:57Z
2016-02-09T13:40:57Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64244
 
Language en
 
Format application/pdf
 
Publisher CCSHAU