MOLECULAR MARKER ANALYSIS BY DNA SEQUENCING AND ESTABLISHING ASSOCIATIONS FOR GRAIN ZINC CONTENT IN RICE (Oryza sativa L.)
KrishiKosh
View Archive InfoField | Value | |
Title |
MOLECULAR MARKER ANALYSIS BY DNA SEQUENCING AND ESTABLISHING ASSOCIATIONS FOR GRAIN ZINC CONTENT IN RICE (Oryza sativa L.)
|
|
Creator |
SUMANTHA HOLLA, K M
|
|
Contributor |
SHASHIDHAR, H E
|
|
Subject |
zinc, planting, grain, vegetative propagation, rice, genotypes, genes, developmental stages, biological phenomena, genetics
|
|
Description |
Zinc deficiency is the second most spread nutrient deficiency worldwide. Rice is the major source of all nutrients for the population in India but it is deficient in Zn. An effort was made to study the effect of nucleotide variations in genes associated with Zn. 20 genotypes contrasting for grain Zn were selected and grown in pots at UAS, GKVK in CRD, during Summer-2013. PCV and GCV were highest for number of tillers, number of productive tillers, grain yield, root length, root number, root volume, grain Zn, shoot Zn and root Zn. Highly positively significant correlation was observed for grain yield with number of productive tillers and productivity per day per plant. Literature survey revealed genes belonging to families like OsZIP, OsYSL, OsNRAMP are involved in transporting Zn. Primers were designed specific to these genes. Single-marker analysis of the primer profile at gel level revealed that OsZIPc was significantly associated to shoot Zn. Sequencing the amplicons of primers OsNRAMP, OsYSL2b, OsZIP3b, OsZIP4a, OsZIP5b and OsZIP6c revealed that variations were present in all these amplicons but, nucleotide changes at seven positions of OsZIP6c were significantly associated with shoot Zn content and nucleotide changes at four positions of OsZIP5b were significantly associated with grain Zn. Although OsZIP5b was monomorphic at gel level, nucleotide variations were significantly associated with grain Zn content. Hence any monomorphic marker may have information as much as a polymorphic marker. The markers identified in this study can be used for improving the grain Zn in rice. July |
|
Date |
2016-11-11T10:40:27Z
2016-11-11T10:40:27Z 2013-08-23 |
|
Type |
Thesis
|
|
Identifier |
http://krishikosh.egranth.ac.in/handle/1/85042
|
|
Language |
en
|
|
Format |
application/pdf
|
|
Publisher |
University of Agricultural Sciences GKVK, Bangalore
|
|