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Genetic analysis and molecular mapping of white rust resistance gene(s) in Brassica spp

KrishiKosh

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Title Genetic analysis and molecular mapping of white rust resistance gene(s) in Brassica spp
 
Creator VIGNESH, M.
 
Contributor D. K. Yadava
 
Subject diseases, fungi, genes, planting, genetics, segregation, crossing over, dna, albugo candida, biological phenomena
 
Description t-8143
tard (B. juncea), which can cause yield losses upto 89.8% under severe conditions. The
most cost effective way of protecting mustard plants from white rust is the introgression of
genetic resistance. A stable resistant donor Bio-YSR (developed by NRCPB, IARI), is
employed as a source of resistance, but it is yet to be genetically characterized. In addition,
some genotypes of B. carinata are also known to show immunity to this disease. Since new
sources of white rust resistance would always be useful for diversification and durability of
resistance, the present study was undertaken to study inheritance and the genetic relationship of
two different resistant sources i.e., in B. juncea and B. carinata for their use in future breeding
programme for white rust resistance. In addition an attempt was also made to identify
microsatellite (SSR) markers for their use in marker assisted selection for white rust resistance.
The inheritance pattern of resistance genes in donors when crossed with two highly
susceptible cultivars, Varuna and Bio-902 (Pusa Jai Kisan), indicated the presence of
monogenic dominant gene for white rust resistance. Cross between the resistant sources from
B. juncea and B. carinata segregated in 15:1 (resistant: susceptible) ratio in F2 generation,
indicating the involvement of different dominant genes governing resistance in these species,
functioning in a duplicate interaction. Hence, the major gene governing white rust resistance
could be easily transferred to well adapted, high yielding and susceptible genotypes by
backcross breeding. The presence of different resistance genes for white rust in the two
different resistant sources will be of immense use in breeding for durable resistance by the way
of diversification of resistant sources and gene pyramiding.
In this study, F2 population derived from the cross between white rust susceptible
(Varuna) and white rust resistant (Bio-YSR) B. juncea lines were evaluated for resistance to A.
candida race 2. Based on screening of 175 SSR primers employing Bulked Segregant Analyses
(BSA), thirty four SSR primers differentiated the two parents and were informative, but none
of them could distinguish the resistant and susceptible bulks. This emphasizes the need to study
further markers for identification of tightly linked markers which can be used for markerassisted
selection for white rust resistance.
 
Date 2016-12-22T09:20:05Z
2016-12-22T09:20:05Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/91984
 
Format application/pdf
 
Publisher iari, DIVISION OF GENETICS