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Molecular Identification of Campylobacter Jejuni and Coli from Chicken, Calves and Dogs to Determine Its Potential Threat on Human Being

KrishiKosh

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Title Molecular Identification of Campylobacter Jejuni and Coli from Chicken, Calves and Dogs to Determine Its Potential Threat on Human Being
Vet World 8(12): 1420-1423
 
Creator Begum, Sonuwara
Sekar, M.
Gunaseelan, L.
Gawande, Monica
Suganya, G.
Malar, P. Annal Selva
Karthikeyan, A.
 
Subject Emerging
Pathogen
Thermophilic Campylobacter
Zoonotic
 
Description Aim: Campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species.The aim of this study was to identify Campylobacter jejuni and Campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (PCR).
Methodology: A total of 104 number of samples comprising cloacal swab from poultry (38), a rectal swab from dogs (40), and calves (26) were collected for the isolation of thermophilic Campylobacters using conventional culture method. PCR was used for identification of mapA gene for C.jejuni and ceuE for C.coli.
Results: The overall presence of Campylobacter was found to be 67(64.42%) from the samples, out of which 6 isolates belongs to C. jejuni species, were 5(18.51%) from chicken and 1(4.17%) from dog was recorded and about 17 isolates belongs to C. coli species were 9(33.33%), 6 (25%), and 1(9.09%) from chicken, dog and calves was recorded.
Conclusion: Results suggested that Campylobacter reservoirs chicken, calves and pet dogs can play a role as the source of infection to human beings and PCR can be an ideal tool for molecular confirmation at the species level.
 
Date 2016-06-13T15:00:56Z
2016-06-13T15:00:56Z
2015-12
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67295
 
Language en
 
Format application/pdf
 
Publisher Veterinary World