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Evaluation and Characterization of Cyanobacteria for Plant Growth Promoting Activity

KrishiKosh

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Title Evaluation and Characterization of Cyanobacteria for Plant Growth Promoting Activity
 
Creator KRISHNASHIS DAS
 
Contributor Sunil Pabbi
 
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Description T-8503
The present investigation was carried out on fifty cyanobacterial strains isolated
from soils of rice and rice-wheat cropping system from different parts of the country
and maintained in germplasm collection of Centre for Conservation and Utilisation of
Blue Green Algae, Indian Agricultural Research Institute, New Delhi. The isolates were
screened for their ability of growth promotion by evaluating the effect of cell free
supernatant of these cultures on germination and growth of rice (var. Pusa 44) and
wheat (var. Pusa 544) seeds. All the strains had positive effect on seed germination of
both rice and wheat showing variable degree of germination. Sixty-six percent strains
showed 100% germination in case of rice whereas 100% germination was observed
with 72% strains in case of wheat as compared to 70% in control. There was variable
response in terms of root growth, shoot growth and fresh weight of seedling in presence
of cell free supernatant with more pronounced effect on shoot length than root length.
In case of fresh weight of seedlings, the culture filtrate was more effective in wheat
seedlings where 80% strains resulted in enhanced fresh weight as against only 46% in
case of rice seedlings. Preliminary screening led to the selection of 10 cyanobacterial
strains, which were further characterized in terms of plant growth promoting parameters
viz. nitrogen fixation, P solubilization, auxin production and siderophore production.
Maximum nitrogenase activity in terms of Acetylene Reduction Assay (ARA) was
shown by Hapalosiphon fontinalis (CCC12) followed closely by Nostoc commune
(CCC89) whereas Nostoc sp. (CCC560) recorded the minimum activity. All the cultures
tested showed P- solubilizing activity with maximum activity shown by Anabaena sp.
(CCC360) followed by Nostoc commune (CCCC89). Indole -3- Acetic acid (IAA)
production was observed in all the strains in presence of varying amount of Ltryptophan
with more production at higher concentration of L-tryptophan and gradual
decrease at lower concentration. Nostoc punctiforme (CCC8) showed the maximum
IAA production followed closely by Nostoc commune (CCC89), Calothrix brevissima
(CCC65) and Nostoc punctiforme (CCC25). The organisms also showed siderophore
production with Nostoc commune (CCC89) showing maximum production along with
Nostoc sp. (CCC560).
The investigations have revealed the potential of cyanobacteria as plant growth
regulators besides their role as nitrogenous biofertilizer in tropical rice fields. Nostoc
commune (CCC89) which performed fairly better in overall production of growth
promoting substances can be considered as potential PGP cyanobacteria and can be
evaluated in field trials
 
Date 2016-11-29T11:47:08Z
2016-11-29T11:47:08Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/88066
 
Format application/pdf
 
Publisher IARI, DIVISION OF MICROBIOLOGY