Evaluation and Characterization of Cyanobacteria for Plant Growth Promoting Activity
KrishiKosh
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Title |
Evaluation and Characterization of Cyanobacteria for Plant Growth Promoting Activity
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Creator |
KRISHNASHIS DAS
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Contributor |
Sunil Pabbi
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Subject |
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Description |
T-8503
The present investigation was carried out on fifty cyanobacterial strains isolated from soils of rice and rice-wheat cropping system from different parts of the country and maintained in germplasm collection of Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi. The isolates were screened for their ability of growth promotion by evaluating the effect of cell free supernatant of these cultures on germination and growth of rice (var. Pusa 44) and wheat (var. Pusa 544) seeds. All the strains had positive effect on seed germination of both rice and wheat showing variable degree of germination. Sixty-six percent strains showed 100% germination in case of rice whereas 100% germination was observed with 72% strains in case of wheat as compared to 70% in control. There was variable response in terms of root growth, shoot growth and fresh weight of seedling in presence of cell free supernatant with more pronounced effect on shoot length than root length. In case of fresh weight of seedlings, the culture filtrate was more effective in wheat seedlings where 80% strains resulted in enhanced fresh weight as against only 46% in case of rice seedlings. Preliminary screening led to the selection of 10 cyanobacterial strains, which were further characterized in terms of plant growth promoting parameters viz. nitrogen fixation, P solubilization, auxin production and siderophore production. Maximum nitrogenase activity in terms of Acetylene Reduction Assay (ARA) was shown by Hapalosiphon fontinalis (CCC12) followed closely by Nostoc commune (CCC89) whereas Nostoc sp. (CCC560) recorded the minimum activity. All the cultures tested showed P- solubilizing activity with maximum activity shown by Anabaena sp. (CCC360) followed by Nostoc commune (CCCC89). Indole -3- Acetic acid (IAA) production was observed in all the strains in presence of varying amount of Ltryptophan with more production at higher concentration of L-tryptophan and gradual decrease at lower concentration. Nostoc punctiforme (CCC8) showed the maximum IAA production followed closely by Nostoc commune (CCC89), Calothrix brevissima (CCC65) and Nostoc punctiforme (CCC25). The organisms also showed siderophore production with Nostoc commune (CCC89) showing maximum production along with Nostoc sp. (CCC560). The investigations have revealed the potential of cyanobacteria as plant growth regulators besides their role as nitrogenous biofertilizer in tropical rice fields. Nostoc commune (CCC89) which performed fairly better in overall production of growth promoting substances can be considered as potential PGP cyanobacteria and can be evaluated in field trials |
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Date |
2016-11-29T11:47:08Z
2016-11-29T11:47:08Z 2011 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/88066
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Format |
application/pdf
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Publisher |
IARI, DIVISION OF MICROBIOLOGY
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