Histopathological and Molecular Characterization of Maize Inbred Lines against Maydis Leaf Blight
KrishiKosh
View Archive InfoField | Value | |
Title |
Histopathological and Molecular Characterization of Maize Inbred Lines against Maydis Leaf Blight
M Sc |
|
Creator |
MANJUNATHA C.
|
|
Contributor |
Robin Gogoi
|
|
Subject |
Maize, maydis leaf blight (MLB), Bipolaris maydis, phenotyping, histopathology, photosynthetic rate, transpiration rate, stomatal conductance, trichome density
|
|
Description |
T-8680
Maydis leaf blight (MLB) caused by Bipolaris maydis is one of the most important fungal diseases of maize. A study was conducted for grouping and histopathological characterization of ten Indian maize inbred lines against MLB disease. On the basis of phenotyping, they were categorized as five resistant inbreds viz. SC-7-2-1-2-6-1, SC24 (92)-2-3-2-1-1, DMSC-16-2, V-351-1-4, HKI MBR-139-2 and five susceptible inbreds viz. CM-119, DMSC-28, HKI PC-8, HKI 1040-C2, V341.The leaf hair density was high in resistant genotypes (299.33) and significantly less in susceptible genotypes (61.00). Stomata count was found less (4.20) in resistant genotypes as compared to susceptible genotypes (11.30). Scanning electron microscopy (SEM) revealed more deposition of wax around the stomata of resistant genotypes but less in the susceptible genotypes. A dense layer of mycelial mat of B. maydis was visualized in susceptible genotypes than in resistant genotypes. Combination of SEM and light microscopic studies revealed that B. maydis penetrated maize leaves through stomata and colonized mesophyll cells. Transmission electron microscopy (TEM) clearly showed the destructive effect of the fungal pathogen on the mitochondria and other cellular organelles. Infra Red Gas Analyzer (IRGA) was used to determine the behavior of physiological parameters of the host plants. Photosynthetic rate was more in uninoculated (healthy) plant than in the inoculated (diseased) plants. Under inoculated conditions photosynthetic rate was significantly high in resistant lines than susceptible inbred lines. Overall photosynthetic rate was gradually reduced with the advancement of time. The transpi ration rate (TR) of inoculated plants was higher than the uninoculated plants at 5 DAI, whereas at 40 DAI, it was decreased in both inoculated and uninoculated plants. Under inoculated condition TR was higher in susceptible genotypes. Stomatal conductance (Cs) decreases with the passage of time under inoculated condition. But a differential behavior of Cs was found among the tested maize inbred lines under uninoculated conditions. |
|
Date |
2016-09-08T18:20:38Z
2016-09-08T18:20:38Z 2012 |
|
Type |
Thesis
|
|
Identifier |
http://krishikosh.egranth.ac.in/handle/1/76168
|
|
Format |
application/pdf
|
|
Publisher |
IARI, Division of Plant Pathology
|
|