Record Details

Evaluation of Bivalent Hvt+Sb-1 Marek’s Disease Vaccine in Chicken

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Evaluation of Bivalent Hvt+Sb-1 Marek’s Disease Vaccine in Chicken
 
Creator Shridhara Murthy, T.S.
 
Contributor Veeregowda, B.M.
Krishnamohan Reddy, Y.
Rathnamma, D.
Satyanarayana, M.L.
Suguna Rao
Suryanarayana, V.V.S.
Byregowda, S.M.
 
Subject ---
 
Description Ph.D. Thesis
The present study was undertaken to evaluate superiority of HVT+SB-1 Marek’s
disease vaccine in comparison with HVT vaccine and to determine the optimum thawing
temperature. The highest titre of the vaccine was obtained at a thawing temperature of
35°C for 45 sec followed by thawing at 20°C, 26°C, and 40°C for 45 sec when held on
ice. No plaques were observed at thawing temperature of 45°C for 45 sec. The vaccine
titres were significantly varied among the different thawing temperatures. The vaccine
virus titre declined significantly at 120 min after reconstitution. Both the vaccines
complied the safety norm laid down by OIE terrestrial manual 2010. The protective index
of HVT+SB-1 and HVT vaccine was 96 and 92.6 respectively. The difference in the
percentage of CD8 cells count between HVT+SB-1 vaccinated group and unvaccinated
challenged control group was significant on 8th dpv. HVT+SB-1 vaccinated birds had
more CD8 cells than HVT vaccinated birds significantly on 23rd dpv. The unvaccinated
and challenged control birds had more CD4 cells than the both vaccinated groups. No
significant difference in CD4 cells was noticed between HVT+SB-1 and HVT vaccinated
groups. qPCR revealed HVT+SB-1 vaccinated birds had less relative MDV-1 load than
HVT vaccinated birds indicating the synergistic action of SB-1. Relative load of HVT
between HVT+SB-1 and HVT vaccinated birds was not significant (P>0.05).
Histopathology revealed infiltration of lymphoid cells was limited in the vaccinated
group compared to control birds. Histopathology lesions were fewer and smaller in
HVT+SB-1 vaccinated birds compared to HVT vaccinated birds. Infiltration of sciatic
nerve with lymphoid cells was absent in HVT+SB-1 and HVT vaccinated group. The
study indicated that HVT+SB-1 is a better vaccine than HVT alone against MD.
 
Date 2016-07-22T15:09:29Z
2016-07-22T15:09:29Z
2011-09-26
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69373
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar