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Molecular Mapping and Localization of Stable QTLs for Charcoal Rot Resistance in Sorghum [Sorghum bicolor (L.) Moench]

KrishiKosh

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Title Molecular Mapping and Localization of Stable QTLs for Charcoal Rot Resistance in Sorghum [Sorghum bicolor (L.) Moench]
 
Creator Palamreddy S.Reddy
 
Contributor B.Fakrudin
 
Subject Plant Bio-Technology
 
Description The genetic architecture of charcoal rot resistance and drought tolerance through the
construction of molecular linkage maps and identification of QTLs can expectedly hasten the
development of charcoal rot and drought tolerant sorghum cultivars. A set of 93
Recombinant Inbred Lines (RILs) derived from IS22380 and E36-1 were evaluated for
charcoal rot incidence and its related traits in sick plot at Main Agricultural Research Station,
Dharwad and Regional Agricultural Research Station, Bijapur during rabi 2004. The RILs
displayed highly significant differences in their mean performance for all traits.
A total of 240 RAPD, 262 genomic SSR and 20 genic SSR markers were used and of which
19 RAPD, 62 genomic SSRs and 4 genic SSR markers were found to be polymorphic
between parents. All 85 markers genotyped across RILs and a genetic linkage map was
constructed by using MAPMAKER/EXP b 3.0 in which 80 markers were assigned to A, B,
C, D, E, F, G, H, I and J linkage groups. A total genomic length of 650.3 cM was covered in
this map. QTL analysis by composite interval mapping using QTL Cartographer (v2.5)
indicated five and four QTLs for charcoal rot component traits at Dharwad and Bijapur
locations respectively on A, B, D and I linkage groups. Three QTLs, one each for length of
infection (xtxp297 on B), number of internodes crossed by the fungus (AC13 on A) and per
cent lodging (xtxp343) explaining a phenotypic variance of 9.29, 12.54 and 15.2 per cent
respectively were detected consistently between two locations and are considered to be the
stable across environments.
 
Date 2016-10-18T08:44:08Z
2016-10-18T08:44:08Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80751
 
Format application/pdf
 
Publisher UAS, Dharwad