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Molecular and biochemical basis of fusarium wilt resistance in pigeonpea [Cajanus cajan (L.) Millsp.]

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Title Molecular and biochemical basis of fusarium wilt resistance in pigeonpea [Cajanus cajan (L.) Millsp.]
 
Creator Patil, Vishal Ramchandra
 
Contributor Patel, R.M.
 
Subject planting, application methods, bacteria, biological development, growth promoters, vegetative propagation, foliar application, strawberry (plant), fruits, acidity
 
Description The present investigation on “Molecular and biochemical basis of fusarium wilt resistance in pigeonpea [Cajanus cajan (L.) Millsp.]” was conducted at Department of plant molecular biology and biotechnology,
NAU, Navsari during 2012-2014. The study was undertaken using wilt resistant cultivars: Vaishali, Maruti and susceptible cultivars: T15-15, Bahar. All molecular and biochemical parameters were carried out on 15 days old seedlings, for biochemical studies leaf and root tissues were collected at three stages 0, 4 and 8 days after infection and control plants. For molecular studies, root tissues were collected at four stages time (T) = 2 h, 24 h, 48 h and 72 h after infection (hai) of Fusarium udum and control tissue at every
stage of infection.
In resistant plant, total sugars content in leaf and root drastically reduced, while in susceptible plants pronounced induction was seen as disease progressed. Total phenols content in leaf and root was increased at 4 DAI and then decreased at 8 DAI in resistant cultivars over control, while in case of susceptible cultivars, it was lower at 4 DAI and increased later on at 8 DAI over control. The trend of Malondialdehyde content content was increased in all tested cultivars after the Fusarium udum infection however, the intensity of MDA was higher in susceptible as compared to resistant cultivar. SOD activity was increased in both leaf and root tissues at all stages in resistant cultivars, while it was decreased in susceptible cultivars. In resistant cultivars, CAT activity was decreased in leaf at 4 DAI, and then after increased at 8 DAI (106.13%), while reverse trend was observed in susceptible cultivars. The enzymes activity of GPX, APX, LOX and PPO increased in all cultivars after Fusarium udum infection in both leaf and root tissues, moreover it was higher in resistant cultivars compared to susceptible
cultivars at all stages. PAL activity increased in all cultivars, moreover activity was higher in resistant cultivar at earlier stage of infection (4 DAI), while it was increased at later stage (8 DAI) in leaf and root tissues of
susceptible cultivars. β-1-3glucanase activity was increased after Fusarium udum infection in all cultivars at all stages over control; moreover it was increased higher at earlier stage i.e. 4 DAI in resistant cultivars, while in susceptible pigeonpea cultivars, it was reported higher at later stage i.e. 8 DAI for leaf and root tissues.
POX isoforms expressed highly intense and more in number in resistant cultivars in leaf and root tissues at all stages after infection as compared to susceptible cultivars. SOD isoforms were over expressed in
resistant cultivars at 4 DAI and then, reduced at 8 DAI, while increased in susceptible cultivars at 8 DAI. In case of polyamines putrescine, spermidine and cadaverine were increased in both leaf and root tissues of resistant cultivars of pigeonpea as compared to susceptible cultivars after infection at all stages.
In molecular studies, relative quantification of gene expression showed that higher and earlier expression was reported in all studied genes in resistant cultivars as compared to susceptible cultivars after Fusarium
udum infection. PR2, PR10 and CHS-8 genes expression were at peak level at 48 hai and retained higher in resistant cultivars than that of susceptible cultivars at all stages. IFS1 and F3H genes expression were induced higher at all stages in resistant cultivar, while remain unaltered in susceptible cultivars after infection. LOX gene expression induced earlier at 2 hai and decreased later on in resistant cultivars, while in susceptible cultivars, it was seen unaltered gene expression. Polyamine synthesis genes ADC, SPDS and
SPMS expression level were found to reach a peak level at 24 hai in resistant cultivars, while, very low level of expression was reported in susceptible cultivars.
Overall, activation and stronger induction of ROS-scavenging mechanisms, phenyl propenoid pathway, polyamines metabolism, releasing elicitors and defense gene expression were reported at early stages of
infection in fusarium wilt resistance in pigeonpea.
 
Date 2016-04-28T14:33:18Z
2016-04-28T14:33:18Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65645
 
Language en
 
Format application/pdf
 
Publisher NAU