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Exploring Farm Gate Level Enzyme Production To Cotton Seed And Tapioca Flour

KrishiKosh

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Title Exploring Farm Gate Level Enzyme Production To Cotton Seed And Tapioca Flour
 
Creator Uma Maheswari, D.
 
Contributor Balakrishnan, V.
Viswanathan, K.
Valli, C.
Pandiyan, V.
 
Subject Customised enzyme extract
Whole cotton seed
Tapioca flour
sheep
Enzyme activity
 
Description A study was carried out to customize enzymes specific to whole cotton seed and
tapioca flour while simultaneously exploring the possibility of producing these
customized enzymes at farm gate level using Trichoderma viridae on paddy husk by solid
state fermentation. Whole cotton seed was rich in crude protein (19.31± 0.17%) and ether
extracts (14.73 ± 0.97%) while tapioca flour was rich in crude fibre (15.45±7.22%) and
soluble carbohydrate (78.27±7.01%) content. Hemicellulose was higher in tapioca flour
than that of whole cotton seed. The per cent glucan, xylan and arabinan in tapioca flour
and whole cotton seed were 27.67 ±1.62, 29.40 ±0 .45 and 24.19 ± 1.78 % and 17.49 ±
0.72, 17.45 ± 1.39 and 18.71 ± 1.29 % respectively. The respective levels of individual
enzyme viz. cellulase, xylanase, glucanase, pectinase and amylase identified for whole
cotton seed and tapioca flour were 46.0, 28.0, 1.5, 2.0 and 9500 (U/g) and, 21.0, 18.0, 0.6, 16.0 and 12000 (U/g). The additive effect of these enzyme when combined as mixture
was studied by including five treatments each with six replications in completely
randomised design to arrive at the “customized enzyme mixture” for tapioca flour as
20.63 U of cellulase, 19.25 U of xylanase, 20.63 U of glucanase and 20.0 U of pectinase
and 10800 U of amylase and was referred as “Customised enzyme mixture for tapioca
flour”, while there was no additive effect for whole cotton seed and the “Customised
enzyme mixture for whole cotton seed” was determined to be 46 U of cellulase, 49.6 U of
xylanase, 46 U of glucanase and 42.2 U of pectinase and 9500 U of amylase.
The second experiment for enzyme production by solid state fermentation on
unground / ground paddy husk with / without culture media supplementation was
designed with four treatments of six replicates in each and lead to inconclusive results.
However, it was noticed that even when Trichoderma viridae was inoculated on unground
paddy husk, the enzyme production was reasonably good and therefore further
investigation on Trichoderma viridae inoculated unground paddy husk with or without
media supplementation was carried out since it is easier to adopt at farm gate.
An in vitro degradability trial using crude enzyme extract from unground paddy
husk supplemented with or without media revealed a significant (p
 
Date 2016-05-23T13:22:41Z
2016-05-23T13:22:41Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66217
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University