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Sub-acute Mastitis Associated with Methicillin Resistant Staphylococcus aureus in a Cow: A Case Report

KrishiKosh

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Title Sub-acute Mastitis Associated with Methicillin Resistant Staphylococcus aureus in a Cow: A Case Report
 
Creator Chandrasekaran, Duraisamy
Venkatesan, Periasamy
Tirumurugaan, Krishnasamy Gopalan
Gowri, Balakrishnan
Subapriya, Subramanian
Thirunavukkarasu, Subramanium
 
Subject Beta lactamase
blaZ
Bovine mastitis
mecA
PCR
Staphylococcus aureus
 
Description A 5-year old Holstein Friesian cross breed cow was presented to Madras Veterinary College Teaching Hospital with the history of reduced milk yield. Clinical examination of udder revealed normal milk color and soft udder. The milk pH was 7.0, with California Mastitis Test score 3+, Electrical Conductivity 270U, and Somatic Cell Count as 328,000. Isolation and identification of causative agent revealed Methicillin Resistant Staphylococcus aureus (MRSA) from the sub-acute mastitis sample. Agar disc diffusion method for antimicrobial susceptibility revealed that the MRSA was sensitive to Enrofloxacin, Gentamicin, Oxytetracycline and Amoxicillin+Sulbactam. On the other hand, the isolate was resistance to Amoxicillin, Penicillin G, Ceftriaxone and Methicillin. The isolate was positive for β-lactamase resistance by Nitrocefin test. The MRSA was confirmed for the presence of mecA and blaZ target genes by polymerase chain reaction (PCR). The cow was treated with Enrofloxacin, Vitamin E and inorganic Selenium, and was recovered after 5 days of post-treatment.
 
Date 2016-06-14T14:57:49Z
2016-06-14T14:57:49Z
2014-12
 
Type Article
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67372
 
Language en
 
Format application/pdf
 
Publisher Journal of Advanced Veterinary and Animal Research