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Development of a laboratory animal model for studies of immunokinetics of peste des petits ruminants virus (PPRV)

KrishiKosh

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Title Development of a laboratory animal model for studies of immunokinetics of peste des petits ruminants virus (PPRV)
 
Creator Lather, Anshul
 
Contributor Sharma, Ravindra
 
Subject PPR, PPRV, Pathogenesis, Real time qRT-PCR, Virus isolation, LPA, HI test
 
Description Peste des Petits Ruminants (PPR) is an acute and highly contagious disease of small ruminants
characterized by high fever, necrotic stomatitis, catarral inflammation of ocular and nasal mucosa,
pneumonia and severe enteritis with high morbidity and mortality. The current understanding of peste
des petits ruminants virus (PPRV) pathogenesis has been mostly assumed from the closely related
morbilliviruses as the available information on this aspect is meager. The lack of a suitable laboratory
animal model has impeded progress of understanding how PPRV causes disease and the role of cell
mediated immunity against this virus. In the present study, rabbit was used as a laboratory animal
model for studies of immunokinetics of PPRV. PPRV Sungri/96 strain was used to infect rabbits via
I/N and I/M routes. All the rabbits remained apparently healthy following infection and no clinical
signs including thermal reaction were observed. Regardless of the route of inoculation, PPRV RNA
was detected intermittently in nasal, rectal and blood samples of infected rabbits mostly between 4 and
8 dpi employing real time qRT-PCR. However, attempts to isolate PPRV from the afforsaid samples in
Vero cells were not successful. The cell mediated immune response of rabbits against PPRV and
Phytohemagglutinin (PHA) was assessed in vitro by lymphocyte proliferation assay (LPA) using MTS
dye. The mean proliferative response against PHA was evident and significantly higher (P
 
Date 2016-08-17T10:51:49Z
2016-08-17T10:51:49Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72688
 
Language en
 
Format application/pdf
 
Publisher LUVAS