CHARACTERIZATION OF INDOLE ACETIC ACID BIOSYNTHESIS IN SELECTED PLANT GROWTH PROMOTING RHIZOBACTERIA OF WHEAT
KrishiKosh
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Title |
CHARACTERIZATION OF INDOLE ACETIC ACID BIOSYNTHESIS IN SELECTED PLANT GROWTH PROMOTING RHIZOBACTERIA OF WHEAT
Ph. D |
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Creator |
MANJUNATH, M.
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Contributor |
Radha Prasanna)
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Subject |
bacteria, planting, iaa, biological development, cyanobacteria, productivity, amino acids, vegetative propagation, acidity, wheats
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Description |
T-8612
Microorganisms inhabiting the rhizosphere of plants are well known for production of auxins, particularly indole-3-acetic acid which plays a significant role in plant growth promotion. The present study was undertaken to evaluate the role of tryptophan and aeration on IAA production by a set of rhizobacteria. Two bacterial strains WRB4 (Providencia sp.) and WRB10 (Alcaligenes sp.) exhibited highest IAA production and enhanced germination, growth of coleoptile and radicle of wheat. Among the cyanobacterial strains, WRC3 (Anabaena oscillarioides) and WRC4 (Anabaena torulosa) exhibited significantly higher IAA in the absence of tryptophan and performed better in seed germination assay. The hydroponic experiment revealed that seed imbibition with WRB4, followed by WRC3 and combinations of WRC4 + WRB4 were most promising in terms of plant growth parameters. Thin layer chromatographic analyses followed by HPLC further confirmed IAA production by the cultures in media and the hydroponic experiment. This is a first time report on IAA production and plant growth promotion in wheat by bacteria belonging to the genera Alcaligenes and Providencia and their synergistic interactions with cyanobacteria. |
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Date |
2016-09-26T19:24:28Z
2016-09-26T19:24:28Z 2010 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/78992
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Format |
application/pdf
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Publisher |
IARI, DIVISION OF MICROBIOLOGY
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