STUDIES ON Choanephora TWIG BLIGHT (Choanephora cucurbitarum (Berk & Rav.) Thaxt. OF CHILLI (Capsicum frutescens L.) AND ITS MANAGEMENT
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Title |
STUDIES ON Choanephora TWIG BLIGHT (Choanephora cucurbitarum (Berk & Rav.) Thaxt. OF CHILLI (Capsicum frutescens L.) AND ITS MANAGEMENT
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Creator |
CHANDRAKALA, J
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Contributor |
VIDYASAGAR, B
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Subject |
diseases, fungi, biological development, choanephora cucurbitarum, biological phenomena, planting, developmental stages, chillies, polysaccharides, pathogens
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Description |
Choanephora cucurbitarum is a facultative saprobe that belongs to the Sub division Zygomycotina, Order: Mucorales and Family Choanephoraceae and it is responsible for causing twig blight in chilli. It is a fungal plant pathogen and has a wide host range (Abel Motaal et al., 2010). The disease was first reported by Dastur (1920) in India and it became endemic in certain monocropping areas. A little is known about chilli twig blight, hence the present investigation has been initiated to study the symptoms and management of disease. A preliminary survey was conducted in 6 major chilli growing districts of Telangana district representing 13 mandals and 38 villages during kharif 2013-14 for the prevalence of Choanephora twig blight diseases in chilli. Khammam recorded maximum disease incidence varying from 20 to 32% in all five villages surveyed where as in Karimnagar and Warangal district the disease incidence was 18 and 15% respectively. There was no incidence of twig blight disease in chilli in Medak, Ranga Reddy and Nizambad districts. The pathogen was isolated from the leaf and proved for its pathogenecity. The drooping multisporous sporangia were subglobose in shape and 90.15 - 100 μm in size. The sporangia were non columellate and dehisce into two half releasing the spores. Sporangiospores were elliptic, fusiform or ovoid in shape, light brown or dark brown in color and are characterized by longitudinal striatures on the surface of wall and 12-19 x 7.23-9.5 μm by size. The sporangiophore (conidiophore) from which the monosporous sporangiola arose was long slender, branched at the apex with primary vesicle from which secondary vesicles were produced on the stalks which bears sporangiospores (conidia). Monosporous sporangiola were elliptical, fusiform or ovoid, striate and measured 11-19 × 4-12 μm. Zygospores of C. cucurbitarum appear not to have been observed prior to the present study. The mycelial and morphological characters were similar to that described by Wolf, 1917. On the basis of morphological and cultural characters, growth habitat and temperature relations, the fungus was identified as Choanephora cucurbitarum. The pathogen restricts its attack on young green twigs as well as petiole, fruit stalk and flower bases. The affected areas on twig first develop as water soaked, brown coloured lesion which enlarge rapidly encircling the twig. The portion of twig above the affected region becomes wilted, blighted and dried up. The fungus develops stiff thread like sporangiophores with black headed asexual reproductive structures on diseased lesions. Prominent hairy growth of Choanephora cucurbitarum on the infected tissue was found in the morning hours. This is visible with a hand lens. Usually whitish mycelia and monosporous sporangial were produced on the lesions. The disease gradually spreads to more and more branches even to the stem causing severe damage under warm and humid conditions that favor the disease development. Studies on the effect of different pH, media and temperature on the radial growth were carried out. The suitable pH for mycelial growth of C. cucurbitarum was at pH 4.5. Among the eight media tesed, PDA was found more suitable for growth of C. cucurbitarum. The temperature of 25 o C was found favourable for the radial growth of C. cucurbitarum. Four isolates of Trichoderma spp and 8 fungal antagonists isolates were isolated from rhizosphere and phylloplane of chilli respectively and tested the efficacy against test pathogen Choanephora cucurbitarum by dual culture technique. Among these, Trichoderma viride isolate-1 was found most effective in inhibiting the test pathogen followed Fusarium spp. Seven fungicides viz., azoxystrobin, carbendazim, captan, copper oxychloride, chlorothalonil, hexaconazole and mancozeb were tested at six concentrations i.e. 0.05, 0.1, 0.15, 0.2, 0.25 and 0.3% respectively under in vitro conditions. Among them captan at 0.15% at least concentration inhibited the mycelia growth of Choanephora cucurbitarum. Ten varieties were screened against the test pathogen C. cucurbitarum and out of them LCA-334 and G-4 showed disease severity index with 27 and 24 per cent respectively. The potential biocontrol agent and effective fungicide were screened under greenhouse conditions against twig blight of chilli caused by Choanephora cucurbitarum with different combinations. The treatment, T2 (captan @ 0.15%) recorded was found to most effective in reducing the disease incidence by 66.47% followed by T7 (Trichoderma viride + captan + Neem oil) 28.9% when compared to control (13.95%). |
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Date |
2017-01-03T10:50:55Z
2017-01-03T10:50:55Z 2016 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/94089
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Language |
en
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Relation |
D10019;
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Format |
application/pdf
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Publisher |
PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABAD
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