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Expression of Immuno- dominant Proteins of O rf virus in Heterologous System and their Diagnostic/ Prophylactic Evaluation

KrishiKosh

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Title Expression of Immuno- dominant Proteins of O rf virus in Heterologous System and their Diagnostic/ Prophylactic Evaluation
 
Creator Yogisharadhya R.
 
Contributor V. Bhanuprakash
 
Subject Unable to Generate Tags majhi thesis.doc
 
Description awt-197
Orf is a zoonotic, highly contagious skin disease affects sheep and goats with a worldwide
distribution. It is caused by orf virus, the prototype of genus Parapoxvirus of the Poxviridae
family. Orf is endemic in most parts of India resulting in significant economical loss. However,
limited attempts were made in the past to characterize the ORFV strains as well as its proteins
to develop new generation diagnostics/prophylactics to control the disease and exploit
immunomodulatory orf viral proteins for broader applications against infectious diseases of
livestock.
In the present study, epidemiological studies of natural orf outbreaks over past one
decade (2001-2012) were done, which showed presence of ORFVs in different agro-climatic
regions, sometimes as a mixed infection both in sheep and goats. Phylogenetic analysis based
on nucleotide/amino acid sequences of A32, B2L and F1L genes demonstrated that Indian
ORFVs are related to each other and to other world wide ORFVs. On the basis of bioinformatics
and structural analysis by homology modelling, conservation of functional motifs
and species specific heterogeneity of A32, B2L and F1L genes were noted, which can be
exploited for molecular epidemiological studies and development of diagnostics. Recombinant
B2L and F1L proteins expressed in E. coli found to be immunogenic and to have potential as
subunit vaccine as assessed by SNT, CIE and ELISA. Newly developed Indirect ELISAs
based on recombinant B2L and F1L proteins showed high sensitivity and specificity for detecting
anti-ORFV antibodies in sheep and goat sera. Immunization of mice with rB2L with or without
subunit antigens of bacterial/viral origin, found to elicit both non-specific as well as specific
protective immunity against virulent challenge. Recombinant B2L protein was also found to
possess antibacterial and lipase activity in addition to molecular adjuvant property indicating
its diverse applications including potential diagnostic, prophylactic and therapeutic utility in
veterinary medicine.
 
Date 2017-01-06T14:42:43Z
2017-01-06T14:42:43Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94861
 
Format application/pdf