Immunohistochemical and In-Vitro Studies on Stage Dependent Fas Mediated Apoptosisin Ovarian Follicles of Buffalo (Bubalus Bubalis)
KrishiKosh
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Title |
Immunohistochemical and In-Vitro Studies on Stage Dependent Fas Mediated Apoptosisin Ovarian Follicles of Buffalo (Bubalus Bubalis)
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Creator |
Rahila Gulzar
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Contributor |
Jamuna, K.V.
Ravindra, J.P. Prasad, R.V. Murthy, V.C. |
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Subject |
sowing, genotypes, fruits, planting, sowing date, developmental stages, beans, yields, seed treatment, inorganic acid salts
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Description |
PG Thesis
The total number of primordial and Graafian follicles is very low in buffaloes and the rate of atresia is also high. Follicular development and atresia depend upon a sophisticated balance between survival and atretogenic factors. Hence the study was undertaken to localize the Fas antigen in different classes of buffalo (Bubalus bubalis) ovarian follicles and to study the effect of FasL in the granulosa cells function. Expression of Fas in different sizes of ovarian follicles of buffalo was studied by Immunohistochemistry. In primordial follicles, Fas expression was detected in most of the atretic oocytes and also in few healthy oocytes also. Oocytes of all the primordial atretic follicles were consistently immuno-positive as were oocytes and granulosa cells of primary and secondary follicles. All the atretic follicles, showed positive reaction and the intensity of staining varied with the degree of atresia. In antral follicles, the expression was mainly seen in granulosa cells. Follicles in the late atretic stage or highly atretic follicles showed more intense positive reaction. Staining was also seen in theca cells but the intensity was less as compared to granulosa cells. In the long term buffalo granulosa cells culture, addition of Fas-L (10ng/ml) significantly (P |
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Date |
2016-10-28T14:40:51Z
2016-10-28T14:40:51Z 2011-07-04 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/82790
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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