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Immunohistochemical and In-Vitro Studies on Stage Dependent Fas Mediated Apoptosisin Ovarian Follicles of Buffalo (Bubalus Bubalis)

KrishiKosh

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Title Immunohistochemical and In-Vitro Studies on Stage Dependent Fas Mediated Apoptosisin Ovarian Follicles of Buffalo (Bubalus Bubalis)
 
Creator Rahila Gulzar
 
Contributor Jamuna, K.V.
Ravindra, J.P.
Prasad, R.V.
Murthy, V.C.
 
Subject sowing, genotypes, fruits, planting, sowing date, developmental stages, beans, yields, seed treatment, inorganic acid salts
 
Description PG Thesis
The total number of primordial and Graafian follicles is very low in buffaloes and
the rate of atresia is also high. Follicular development and atresia depend upon a
sophisticated balance between survival and atretogenic factors. Hence the study was
undertaken to localize the Fas antigen in different classes of buffalo (Bubalus bubalis)
ovarian follicles and to study the effect of FasL in the granulosa cells function.
Expression of Fas in different sizes of ovarian follicles of buffalo was studied by
Immunohistochemistry. In primordial follicles, Fas expression was detected in most of
the atretic oocytes and also in few healthy oocytes also. Oocytes of all the primordial
atretic follicles were consistently immuno-positive as were oocytes and granulosa cells of
primary and secondary follicles. All the atretic follicles, showed positive reaction and the
intensity of staining varied with the degree of atresia. In antral follicles, the expression
was mainly seen in granulosa cells. Follicles in the late atretic stage or highly atretic
follicles showed more intense positive reaction. Staining was also seen in theca cells but
the intensity was less as compared to granulosa cells.
In the long term buffalo granulosa cells culture, addition of Fas-L (10ng/ml)
significantly (P
 
Date 2016-10-28T14:40:51Z
2016-10-28T14:40:51Z
2011-07-04
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82790
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar