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ESTIMATION OF GENETIC DIVERSITY IN RICE ACCESSIONS EMPLOYING MOLECULAR MARKERS AND DROUGHT TOLERANCE TRAITS

KrishiKosh

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Title ESTIMATION OF GENETIC DIVERSITY IN RICE ACCESSIONS EMPLOYING MOLECULAR MARKERS AND DROUGHT TOLERANCE TRAITS
 
Creator SURENDRA, THUMMALA
 
Contributor LAKSHMI NARAYANA REDDY, V
 
Subject genetics, rice, genotypes, vegetative propagation, tolerance, weather hazards, dna, biological phenomena, drying, genetic structures
 
Description Rice (Oryza sativa L.) is the main staple food crop of Asia and is being
consumed by more than half of the population of the developing countries in the
world. Genetic diversity is pre-requisite for any crop improvement programmes as
it helps in the development of superior recombinants and selection of parents
having wider variability for different characters. Therefore, there is a need to
diversify the genetic base of improved rice cultivars. The present work was
initiated to evaluate the available rice accessions at both drought tolerance and
molecular level. To this end, 30 rice genotypes both drought tolerant and
susceptible were included. These genotypes have been screened with 50 hyper
variable microsatellite markers to estimate the genetic diversity of the genotypes.
123 alleles were detected among all the rice accessions using 46 primer pairs with
an average of 2.7 alleles per microsatellite locus, which was sufficient to classify
the rice accessions investigated. In addition, they were also screened for their
drought tolerance using PEG-induces stress and data of root and shoot related
traits viz., Root length, Root dry weight, Shoot length, Shoot dry weight, Root
shoot weight ratio, Relative shoot length, Relative root length, Relative shoot dry
weight, Relative root dry weight, and Relative root-shoot weight ratio were
taken. The genetic diversity of the rice genotypes is being estimated using both
molecular data as well as drought related traits. This work is expected to give
more insights in selection of parents for development of drought tolerant varieties
that is the need of the hour. The results of the present study gives useful
information regarding the selection of suitable parents in breeding of drought
tolerant rice cultivars besides useful in mapping of important QTLs related to
drought tolerant traits. The molecular markers liked to the QTLs could be of value
in selection of lines with drought tolerance.
Using of varieties tolerant to different stresses in breeding programmes can
bring out useful transgressive segregants with increased tolerance to more than
one stress. The results reported here have significant implications for rice
breeding and basic studies as well. Closely related or moderately distant parents
can be chosen based on the overall grouping reported here and depending on the
breeding objectives. The two flood-tolerant varieties which grouped with drought
tolerant varieties are likely sources of new alleles for salt tolerance.
 
Date 2016-06-23T09:09:13Z
2016-06-23T09:09:13Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67765
 
Language en
 
Relation D9134;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY