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Studies on methylotrophs and their beneficial effects on soybean (Glycine max (L.) Merrill)

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Title Studies on methylotrophs and their beneficial effects on soybean (Glycine max (L.) Merrill)
 
Creator T.K.Radha
 
Contributor V.P.Savalgi
 
Subject Agricultural Microbiology
 
Description Investigations were carried to study the Methylotrophs and their beneficial effects on
soybean. Samples were collected from Dharwad and Belgaum districts of Karnataka; about
forty isolates were isolated on selective AMS medium. Further the isolates were
characterized based on their morphological and biochemical characteristics. The results
revealed that all the isolates were Gram negative, rod shaped with the dimension of 0.6-1.0 x
1.0-1.5 μm, accumulate PHB, colonies were pink, pale pink or dark pink in colour due to
pigmentation. Biochemical characteristics revealed that all the isolates showed positive
results for oxidase test, urease test, indole production. None of the isolates showed positive
results for casein hydrolysisn, hydrogen sulphide production, MR and VP test, nitrate
reduction test, all the isolates showed positive results for utilization of glucose, D-galactose,
dichloromethane, chlorofrom and glycerol.
Further the isolate were screened for beneficial characters. The results revealed that
the highest Indole acetic acid production was recorded in ML55 (28.15 μg/ml of culture
filtrate) the maximum GA production of 70.27 μg/ml of culture filtrate was observed in
ML55, the highest nitrogen fixation was observed in ML2 (1.187 mg N/g of malate medium).
The isolates were also tested for cytokinin production, siderophore production and Psolubilization;
the results revealed that the maximum zeatin production was recorded in ML2
(10.1 ng/ml of culture filtrate). The maximum siderophore production as recorded in ML2
(0.60 μ moles of a-2, 3 di hydroxy benzoic acid). But none of the isolates were able to
solubilize phosphate. Based on these beneficial characters three efficient isolates were
selected for pot culture experiment along with Bradyrhizobium japonicum strain SB120.
Results revealed that compared to single inoculation treatment combined inoculation
treatment performed better, among combined inoculation treatment T9 (Reference strain + B.
japonicum strain SB120 and T7 (ML55+B. japonicum strain SB120) recorded higher growth and
yield parameters.
 
Date 2016-10-17T11:25:50Z
2016-10-17T11:25:50Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80680
 
Format application/pdf
 
Publisher UAS, Dharwad