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Molecular characterization of inter-specific backcross inbred lines of rice (Oryza sativa L.) for mapping of yield component QTL

KrishiKosh

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Title Molecular characterization of inter-specific backcross inbred lines of rice (Oryza sativa L.) for mapping of yield component QTL
 
Creator Bhatia, Dharminder
 
Contributor Singh, Kuldeep
 
Subject yields, rice, genomes, genetics, planting, seasons, grain, genotypes, developmental stages, genes
 
Description We need to significantly increase the yield potential of rice for feeding estimated 9 billion
populations by 2050. For increasing yield potential in rice, utilization of wild species is one
among the several strategies advocated. In the present study, Backcross inbred lines (BILs)
derived from O. longistaminata acc. IRGC104301 (longi-BILs), O. rufipogon acc. IRGC104433
(rufi-BILs) and O. glumaepatula acc. IRGC104387 (glumae-BILs) in the background of O. sativa
ssp. indica cv. PR114 were used for mapping QTLs consistently contributing variation for yield
and yield component traits. BILs were evaluated for yield and yield component traits as
compared to recurrent parent PR114 as check in alpha lattice design over three seasons spanning
two locations. A modified Genotyping by sequencing approach was used for genotyping.
Sequence data for each population was analysed with custom designed method exactly similar for
each population to identify SNPs. A total of 3322 informative SNPs of rufi-BILs and 3437
informative SNPs of glumae-BILs were used for mapping QTLs for twelve yield and yield
component traits using inclusive composite interval mapping. SNPs identified in longi-BILs
could not be used for mapping due to large number of missing data points. In rufi-BILs, QTL for
thousand grain weight (qtgw5.1) was mapped on chromosome 5 consistent over all the three
seasons and with positive additive effect contributed by O. rufipogon allele. Two QTLs for grain
width (qgw5.1 and qgw5.2) were also mapped on chromosome 5 with positive and negative
additive effect respectively. In glumae-BILs, three consistent QTL for thousand grain weight on
chromosome 2 (qtgw2.1), 3 (qtgw3.1) and 6 (qtgw6.1) were mapped consistently for season 2 and
3. QTLs qtgw2.1 and qtgw6.1were associated with positive additive effect, while qtgw3.1 with
negative additive effect contributed by O. glumaepatula allele. Two QTL for grain length (qgl7.1
& qgl7.2) with positive additive effect contributed by PR114 allele was mapped on chromosome
7 spanning overlapping position in both rufi-BILs and glumae-BILs respectively. qgl7.1 and
qgl7.2 might be same QTL as contributed by same parent and spanning overlapping position.
QTL for other yield and yield component traits could not be identified due to skewed
segregation.Yield component QTLs identified in the present study from low yielding wild
relatives of rice reveals their significance in improving yield of cultivated rice. Combining
these QTLs in the background of cultivated rice will not only increase the yield, but also widen
the genetic base of cultivated rice.
 
Date 2016-04-18T12:21:16Z
2016-04-18T12:21:16Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65474
 
Language en
 
Format application/pdf
 
Publisher PAU