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Identification and cloning of Avr: Pi-kh gene from Magnaporthe oryzae

KrishiKosh

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Title Identification and cloning of Avr: Pi-kh gene from Magnaporthe oryzae
 
Creator Soham Ray
 
Contributor T. R. Sharma
 
Subject genes, proteins, amino acids, cloning, biological phenomena, rice, genomes, planting, fungi, acidity
 
Description T-8103
Rice is one of the most important cereal crops which play a significant role in achieving
global food security. However, it suffers from several biotic and abiotic stresses that
seriously affect its production. Among all the biotic stresses, rice blast caused by
Magnaporthe oryzae, is the most devastating disease which results in heavy yield loss
wherever this crop is grown. A dominant blast resistance gene, Pi-kh
, present in indica
rice line ‘Tetep’ exhibit resistance to M. oryzae isolates prevalent in various parts of
India has already been cloned and characterized. In the present investigation attempts
were made to identify probable candidates of Avr: Pi-kh gene in silico and to clone those
candidates from M. oryzae strain PLP-1 which is incompatible on rice blast resistance
gene Pi-kh
.
In this investigation, a total of 474 candidate Avr-genes were identified in
the 37.878 Mb genome of M. oryzae strain 70-15. Of these 25 candidates were used for
in silico protein modeling and performing protein-protein interaction with blast
resistance gene Pi-kh
. Based on this study two candidate genes i.e., Avr: Pi-kh
-1 and Avr:
Pi-kh
-2, were cloned and characterized from M. oryzae strain PLP-1. In silico expression
analysis, interaction study with Pi-kh gene, and annotation studies showed that these two
Avr genes would be the perfect counterparts of blast resistance gene Pi-kh
. The results
obtained in this study have practical implications. Since, the Pi-kh
gene is already
recognized and cloned, the Avr: Pi-kh gene, once validated, could be used in R-Avr two
components system to develop broad spectrum resistance against rice blast disease.
Beside these, the in silico approach followed in this study to clone a gene might prove as
an alternative strategy to clone more fungal Avr genes.
 
Date 2016-12-16T12:05:38Z
2016-12-16T12:05:38Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/90550
 
Format application/pdf
 
Publisher IARI, Division of Molecular Biology and Biotechnology