CELL WALL DEGRADING ENZYMES OF Fusarium solani (Mart.) Sacc. INCITANT OF DECLINE OF Piper betle.
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Title |
CELL WALL DEGRADING ENZYMES OF Fusarium solani (Mart.) Sacc. INCITANT OF DECLINE OF Piper betle.
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Creator |
SANTHA LAKSHMI, B
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Contributor |
Balasubramanian, K.A
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Subject |
Cell, wall, degrading, enzymes, Fusarium, solani,Sacc, incitant, decline, Piper, betle
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Description |
The elaboration of cell wall degrading enzymes of F. solani, incitant of betelvine decline and the role of calcium in the reduction of elaboration of these enzymes we~e studied in the present investigation. The affected betelvine root sections showed brown vascular discolouration. Fungal mycelium was also observed in the xylem vessels. Isolations from those sections yielded Fusarium solani incitant of betelvine decline. Of the two · methods i.e, dipping of intact roots and clipped roots in spore suspension followed to prove pathogenicity, maximum infection of the ·CUttings was observed when the plant roots were clipped before dipping them in spore suspension. In susceptible variety, the activity of polygalacturonase and pectin methyl esterase was more. In moderately resistant and resistant varieties the activity of polygalacturonase and pectin methyl esterase was moderate and less respectively. The application of 5.0 tonnes of gypsum/ha to betelvine plants the activity of polygalacturonase and pectin methyl esterase was less. Less activity of polygalacturonase and pectin ·methyl esterase was observed in plants fed with 2.5 tonnes of gypsum/ha compared to control plants. The activity of polygalacturonase and pectin methyl esterase was low in plants where water was drained compared to the plots for which water was not drained. Pectin trans-eliminase was not detected. The calcium deficient plants showed more activity of polygalacturonase and pectin methyl esterase and more vascular browning. Calcium fed plants showed less of po~ygalacturonase, pectin methyl esterase vascular browning. activity and low The present study confirms that the involvement of Fusarium solani in the pathogenicity of betelvine decline and the help rendered by polygalacturonase and pectin methyl esterase to Fusarium solani to successfully colonize the betelvine plants. Pectin trans-eliminase was not produced by Fusarium solani. Calcium reduced the elaboration of polygalacturonase and pectin methyl esterase. Magnesium content in the soil contributed to the development of decline disease. The polygalacturonase and pectin methyl esterase produced the typical wilting and- vascular browning. |
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Date |
2016-08-17T14:46:22Z
2016-08-17T14:46:22Z 1990 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/72802
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Language |
en
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Relation |
D3385;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD.
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