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IN VITRO PROPAGATION OF Aloe vera L.

KrishiKosh

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Title IN VITRO PROPAGATION OF Aloe vera L.
 
Creator VEENA, DEVI
 
Contributor KANWER, KAMLESH
 
Subject ---
Aloe vera L.”,In vitro propagation
 
Description ABSTRACT
The present investigation aims at “In vitro propagation of Aloe vera L.”
To develop micropropagation protocol shoot bud was used as explant. The
surface sterilization was carried out using 0.2% bavistin and 0.5% sodium
hypochlorite. Maximum per cent establishment of 72.22% was observed on solid
MS medium supplemented with 10 μM BA, 5.0 μM Kinetin and 1.50 μM NAA
during the first week of April. The best medium for the multiplication of
microshoots was solid MS medium supplemented with 10 μM BA and 7.5 μM
Kinetin resulting in per cent multiplication of 69.44%. Maximum number of
microshoot per explant of 22.70 and plant length of 8.99 cm was observed at the
end of sixth subculturing. Highest percentage of in vitro rooting (76.39%),
maximum number of roots (3.33) and root length (3.67) was observed on solid
MS medium supplemented with 0.04 % activated charcoal within 10-15 days.
Presence of activated charcoal showed positive effect on the increase in the
length of plantlets. Microshoots were also rooted ex vitro in sand without any
treatment of growth regulators and rooted within 30 days, however per cent
rooting (23.33%) and root length (1.04 cm) was smaller as compared to roots
generated in vitro. Well rooted plantlets were successfully acclimatized in the
sand in the greenhouse conditions and after 16th week 69.44 % of the plants
survived.
 
Date 2017-01-04T12:36:18Z
2017-01-04T12:36:18Z
2012
 
Type Thesis
 
Identifier 47728
http://krishikosh.egranth.ac.in/handle/1/94397
 
Language es
 
Format application/pdf