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CHARACTERIZATION OF MAIZE GENOTYPES

KrishiKosh

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Title CHARACTERIZATION OF MAIZE GENOTYPES
 
Creator SUDHAKAR NAIDU, D.H
 
Contributor GANESH, M
 
Subject CHARACTERIZATION, MAIZE, GENOTYPES
 
Description In the present investigation 50 maize genotypes were studied for varietal
characterization based on morphological characters, reaction of seeds to
different chemicals, seedling growth response to growth regulators and 24
genotypes were studied based on electrophoretic banding pattern of total
soluble seed proteins. The experiment was conducted at Maize Research Centre
(MRC), Rajendranagar, Hyderabad, during rabi 2006-07 and the laboratory
studies were conducted at Seed Research and Technology Center (SRTC),
Rajendranagar, Hyderabad.
Distinct, Uniform, Stable (DUS) morphological characters were
recorded for fifty genotypes, which were least influenced by growing
conditions. Based on chemical tests and electrophoretic technique, flow charts
were developed, which are useful for field functionaries involved in seed
production/ certification for easy identification and to verify the genetic purity
of seeds of maize genotypes.
The results in the present study clearly indicated that the maize
genotypes can be distinguished and identified by plant/seed morphological
characters. Grouping of the genotypes was accomplished based on chemical
tests viz., seedling growth response to GA3 (low, medium and high), 2,4-D
(less, moderate and high) and seed color reaction to modified phenol (no color,
light brown, dark brown and black).
Electrophoresis of seed proteins was considered reliable and give
accurate, reproducible results for varietal identification in maize .The resultant
electrophoregrams drawn from seed protein electrophoresis (SDS-PAGE)
showed distinct differences in the number of bands viz., minimum number (16)
of bands in 5235 and maximum number of bands (28) in CM 132, intensity of
bands (very light, light, medium and dark) and also in relative mobility values,
ranged from 0.10 to0.91, which enable to characterize the genotypes.
 
Date 2016-08-08T13:36:03Z
2016-08-08T13:36:03Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71504
 
Language en
 
Relation D8151;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD