Record Details

Genetic Analyses of Resistance to Stored Grain Weevil (Sitophilus oryzae L.) in Maize

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Genetic Analyses of Resistance to Stored Grain Weevil (Sitophilus oryzae L.) in Maize
M Sc
 
Creator RAJKUMAR UTTAMRAO ZUNJARE
 
Contributor Firoz Hossain
 
Subject grain, maize, developmental stages, genotypes, genetics, biological interaction, germinability, inbred lines, research methods, plant oils
 
Description T-8635
A diverse set of 230 inbred lines were evaluated to assess the genetic variability for
resistance to stored grain weevil (Sitophilus oryzae). ANOVA revealed significant
variation for (i) grain weight loss (3.40 to 42.32%), (ii) number of insect progeny
emerged (5.00 to 76.33), (iii) germination (0 to 81.33%), (iv) pericarp thickness
(36.18 to 194.83 μm) and (v) grain hardness (62.33 to 1171.67 N) across three
experiments. Grain weight loss and number of insect progeny emerged was positively
correlated, while germination percentage was negatively correlated with grain weight
loss and number of insect progeny emerged. Pericarp thickness and grain hardness did
not show any association with the above mentioned traits. Among the genotypes,
CML394, SKV21, LM13, Pant109, Pant124, CML442, MGB1, CML207, HKI209
and HUZM185 were identified as promising inbreds with resistance against weevil
infestation. These resistant inbreds had less grain weight loss, insect progeny and high
germination percentage. Among the specialty corn types, pop corn inbreds were more
resistant, while QPM inbreds were highly susceptible. The resistant inbred lines thus
identified in the study can serve as the potential donors in the resistance breeding
programme. Diversity analyses using 63 SSR markers among 48 inbred lines differing
for resistance, grouped them into three distinct clusters. A total of 177 alleles were
detected with polymorphism information content ranging from 0.04 (umc1179) to
0.74 (umc182) with mean of 0.44. The genetic dissimilarity of the parental pairs was
found to vary from 0.32 (HKI193-1 and HKI193-2) to 0.77 (HKI193-1 and VQL1),
with average Jaccard’s dissimilarity coefficient of 0.62. The principle coordinate
analysis revealed diverse spatial distribution of inbreds across four quadrangles,
suggesting the presence of genetic divergence among the inbreds. Crosses among
genetically diverse resistant inbreds may be attempted to exploit grain heterosis.
Contrasting (resistant and susceptible) diverse parents can be further crossed to
develop a suitable mapping population for the detection of QTLs for weevil
resistance.
 
Date 2016-09-20T16:52:29Z
2016-09-20T16:52:29Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/77817
 
Format application/pdf
 
Publisher IARI, DIVISION OF GENETICS