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Cryopreservation of Encapsulated Buffalo Bull Semen

KrishiKosh

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Title Cryopreservation of Encapsulated Buffalo Bull Semen
 
Creator Gite, V. N.
 
Contributor Patil, M. S.
 
Subject Animal Reproduction and Gynaecology
Cryopreservation of Encapsulated Buffalo Bull Semen
 
Description The present study on “Cryopreservation of Encapsulated Buffalo Bull
Semen”, was carried out by collecting the semen twice in a week from a total of 6
mature buffalo bulls by artificial vagina method in the month of April – May, 2012
for 3 consecutive weeks. Immediately after collection of semen, sperm
concentration, initial motility, viability, plasma membrane integrity and acrosome
integrity of spermatozoa were accessed. The semen was further divided into two equal parts. Part one was utilized for conventional method of cryopreservation
(Group – I – Control), where as the other half of semen was utilized for the
preparation of encapsulated semen capsules (Group – II - Encapsulation) and its
further cryopreservation,.
At a concentration of 1.5 per cent Sodium Alginate, 1 per cent Calcium
Chloride and 0.01 per cent Ploy – L – Lysine, encapsulated semen capsules of a
spherical shape, having average volume of 4.55 μl containing 3.34 X 106
spermatozoa / capsule were prepared.
In the present study, immediately after collection, the neat buffalo bull
semen had average volume of 3.03 ml and a sperm concentration of 1222.34 X
106 / ml.
An average initial motility of 66.11 ± 2.24 per cent is observed which after
addition of 1.5 per cent sodium alginate solution in Group – II (Encapsulation),
non significantly reduced to 62.92 ± 2.08 per cent. After dilution and equilibration
in Tris-Egg Yolk- Citrate dilutor, non significant difference in Group – I (Control)
and Group II (Encapsulation) for average motility (53.29 ± 2.51 and 49.44 ± 2.12
per cent) of buffalo bull semen was observed, respectively. The post thaw motility
in Group – I (Control) and Group – II (Encapsulation) after 5 minutes equilibration
period before freezing differs significantly from it after 30 and 60 minutes
equilibration period.
Average initial viability of 76.05 ± 2.22 per cent, with subsequent non
significant reduction to 70.80 ± 2.38 per cent after mixing sodium alginate with
buffalo bull semen in Group – II (Encapsulation) was observed in the present
study. Significantly higher (57.57 ± 2.00 per cent) viable spermatozoa are
observed in buffalo bull semen sample diluted in Group – II (Encapsulation) than
in Group – I (Control) 34.10 ± 1.46. After equilibration of buffalo bull semen at
40C for 5, 30 and 60 minutes in dilutor, significantly higher percentage of viable
spermatozoa (56.00 ± 2.54, 54.39 ± 2.95 and 62.31 ± 2.16 per cent) were
observed in Group – II (Encapsulation) than in Group – I (Control) (32.00 ± 2.12,
35.25 ± 1.89 and 35.04 ± 1.90 per cent). After cryopreservation and thawing, in
Group – II (Encapsulation) significantly higher post thaw viability (32.10 ± 1.61
per cent) is observed than in Group – I (Control) (24.28 ± 1.14 per cent).
Plasma membrane intact spermatozoa in the initial buffalo bull semen
samples were 69.74 ± 2.15 per cent, which immediately after the addition of 1.5
per cent sodium alginate solution in Group – II (Encapsulation), showed an
average of 64.80 ± 1.99 per cent. Significantly higher plasma membrane intact spermatozoa (56.18 ± 1.62 per cent) were observed in Group – II (Encapsulation)
than in Group – I (Control) (28.07 ± 1.12 per cent) buffalo bull semen after
dilution and equilibration. In post thawed buffalo bull semen samples
cryopreserved in Group – I (Control) and Group – II (Encapsulation), an average
of 21.28 ± 1.08 and 30.58 ± 1.60 per cent plasma membrane intact spermatozoa
are noted, respectively (P
 
Date 2017-01-03T16:17:53Z
2017-01-03T16:17:53Z
2012-09-16
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94257
 
Language en
 
Format application/pdf
 
Publisher MAFSU