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In Vitro Screening for Salt Tolerance in Cotton

KrishiKosh

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Title In Vitro Screening for Salt Tolerance in Cotton
 
Creator Nirmala
 
Contributor H.M. Vamadevaiah
 
Subject Plant Biochemistry
 
Description A laboratory experiment was conducted during 2009-10 in the ?Tissue culture
laboratory? of Agriculture Research Station, Dharwad Farm, University of
Agricultural Sciences, Dharwad to study the effect of in vitro induction of salt stress
on growth parameters, osmoprotectant and two enzymes belonging to the class of
oxido-reductases in three cotton genotypes viz. Bikeneri Nerma, female parent of
NHH44, a popular cotton hybrid for rain-fed situation, AC-738, male parent of same
hybrid and Jayadhar, a diploid cotton cultivated under rain-fed conditions. The
salinity stress was induced by incorporating different concentrations of salts to
generate EC 2, 4, 6 and 12 referred to as T1 to T5, respectively. The experiment was
laid out in completely randomised design with three replications. Reduction in
seedlings fresh weight, shoot length, root weight, root length and root dry weight was
significantly less in Bikaneri Nerma under salt stress as compared to Jayadhar and
AC- 738. Salinity stress led to significant increase in proline content in shoots and
roots of Bikaneri Nerma when compared to Jayadhar and AC-738. Bikaneri Nerma
showed significantly increased Nitrate reductase (NRA) and Peroxidase (POD) in
shoots from treatments T1 to T5, whereas in roots the activities of NRA and POD
increased up to T2 and T3 onwards the activities declined. The protein expression was
studied by SDA-PAGE in all the three genotypes under control and at EC level of 6.
The Bikaneri Nerma and Jaydhar genotypes expressed extra protein. Polymorphic
difference was also observed among the genotypes. The genotypes studied differed
widely in their response to salinity exhibiting thereby that different genotypes may
have different adaptation levels against salinity stress.The information generated from
the present investigation reveals that the genotypes Bikaneri Nerma and Jayadhar may
be better suited for salinity stress conditions.
 
Date 2016-11-10T15:35:36Z
2016-11-10T15:35:36Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/84928
 
Format application/pdf
 
Publisher UAS, Dharwad