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Genetic diversity of abiotic stress tolerant cluster bean (Cyamopsis tetragonoloba (L.) Taub.) rhizobia

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Title Genetic diversity of abiotic stress tolerant cluster bean (Cyamopsis tetragonoloba (L.) Taub.) rhizobia
 
Creator Subha
 
Contributor Gera, Rajesh
 
Subject Cyamopsis tetragonoloba, PEG, MspI, HaeIII
 
Description Cluster bean (Cyamopsis tetragonoloba (L.) Taub.) locally known as guar, guarpalli etc.
belongs to the family Fabaceae. It is an important legume mainly grown as hardy and drought tolerant
crop. Environmental stress is a major component of natural selection in soil ecosystem. Among several
environmental conditions, the most prominent problematic abiotic factors in the arid regions are
drought and high temperature. For successful functioning of microbial bioinoculants and their influence
on plant growth and soil health, exhaustive efforts have been made to expose microbial diversity, their
distribution and behavior. Studies on molecular diversity of root nodule bacteria in rhizosphere or
nodules have been conducted to classify these rhizobia and also to correlate with symbiotic properties,
effectiveness and competitiveness of these bacteria.
In the present investigation, a total 165 rhizobial isolates were obtained from 67 soil samples
collected from South-West Haryana using trap plant to study the diversity of rhizobia nodulating
clusterbean. All the rhizobial isolates tested were able to grow at all the three temperatures i.e. 30, 35
and 40, except few isolates where growth was slightly suppressed at 40oC. However, only 28 rhizobial
isolates were able to grow at 45oC, out of which some showed good, moderate and poor growth. Most
of rhizobial isolates were able to grow up to 20% PEG. However, there was drastic decrease in their
growth rate with increasing PEG concentration. Only, 54 and 8 rhizobial isolates were able to grow at
30 and 40% PEG, respectively. All the selected rhizobial isolates were also characterized for IAA
production, siderophore production, P-solublization, ACC utilization and molecular characterization.
The genomic DNA of all these rhizobial isolates were isolated by CTAB method and tested for nodC
and nifH gene primers and hence authenticated as rhizobia. Genomic DNA of all the rhizobial isolates
were also amplified with 16S rDNA gene primers using 27F and 1378R primers. The amplified product
was subjected to RFLP analysis with MspI and HaeIII restriction enzymes. Dendrogram based on 16S
rDNA profiles showed lot of diversity. Analysis of cluster bean rhizobial isolates on ARDRA basis, 17
different biotypes were formed at 80% similarity coefficient. Most prevalent biotype was 3rd type,
which prevails in all the three districts. On the basis of abiotic stress tolerance, biochemical and
molecular characterization, three cluster bean rhizobial isolates (GB-36b, GB-26d and GH-2b) were
selected as most efficient rhizobial isolates.
 
Date 2016-09-10T09:24:06Z
2016-09-10T09:24:06Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76343
 
Language en
 
Format application/pdf
 
Publisher CCSHAU